Momoi T, Momoi M Y, Kurata T
J Neurochem. 1986 Jan;46(1):229-34. doi: 10.1111/j.1471-4159.1986.tb12951.x.
Peanut agglutinin receptor, which is a glycoconjugate with terminal Gal(beta 1-3)GalNAc residues, was found to be a specific biochemical marker of the myelin in rat brain. The localization of peanut agglutinin receptor in rat brain was studied by use of fluorescein-conjugated peanut agglutinin. In adult rat brain, receptors were found only on myelin. In contrast, the receptors in fetal brain were not found in the white matter, but only in the ependyma. Peanut agglutinin receptors were extracted from adult and fetal rat brains with 2% Triton X-100, subjected to electrophoresis on 10% sodium dodecyl sulfate-polyacrylamide gel, blotted onto nitrocellulose filters, and detected by their reaction with peroxidase-conjugated peanut agglutinin. A major receptor of Mr 130,000 and a minor one of Mr 200,000 were found in both adult and fetal rat brains.
花生凝集素受体是一种带有末端Gal(β1-3)GalNAc残基的糖缀合物，被发现是大鼠脑髓磷脂的一种特异性生化标志物。利用荧光素偶联的花生凝集素研究了花生凝集素受体在大鼠脑中的定位。在成年大鼠脑中，受体仅存在于髓磷脂上。相比之下，胎儿脑中的受体在白质中未发现，仅存在于室管膜中。用2% Triton X-100从成年和胎儿大鼠脑中提取花生凝集素受体，在10%十二烷基硫酸钠-聚丙烯酰胺凝胶上进行电泳，转移到硝酸纤维素滤膜上，通过与过氧化物酶偶联的花生凝集素反应进行检测。在成年和胎儿大鼠脑中均发现了一个主要的130,000 Mr受体和一个次要的200,000 Mr受体。
