Riggs G H, Schweitzer L
Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, Kentucky 40292.
J Anat. 1994 Oct;185 ( Pt 2)(Pt 2):387-96.
Various studies have suggested that glycoconjugates may influence connectivity and lamination in the developing central nervous system and may function as barriers to neuritic extension. It has been proposed that the peanut agglutinin lectin labels a glycoconjugate subserving a barrier function. We chose to investigate the distribution of this peanut-agglutinin-labelled glycoconjugate in the dorsal cochlear nucleus of the developing hamster since the development of the dorsal cochlear nucleus is well characterised and its axons obey laminar boundaries. The distribution of peanut agglutinin label throughout the cochlear nucleus delineated zones that cochlear axons fail to invade. In the dorsal cochlear nucleus, laminar differences were reduced on postnatal d 13 and virtually disappearing by postnatal d 23. Label in the molecular layer dissipated as axons and dendrites grew into this layer. These patterns of peanut agglutinin binding correspond to axonal ingrowth and are consistent with a barrier function for glycoconjugates in the molecular layer.
多项研究表明，糖缀合物可能会影响发育中的中枢神经系统的连接性和分层，并且可能起到神经突延伸屏障的作用。有人提出花生凝集素标记了一种具有屏障功能的糖缀合物。由于耳蜗背核的发育特征明确且其轴突遵循层界，我们选择研究这种花生凝集素标记的糖缀合物在发育中小仓鼠耳蜗背核中的分布。花生凝集素标记在整个耳蜗核中的分布勾勒出耳蜗轴突未能侵入的区域。在耳蜗背核中，出生后第13天层间差异减小，到出生后第23天几乎消失。随着轴突和树突生长进入分子层，分子层中的标记消失。这些花生凝集素结合模式与轴突向内生长相对应，并且与分子层中糖缀合物的屏障功能一致。
