Liu Zhenzhen, Zhao Xiao, Bangash Hina Iqbal
Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu University, Chengdu, China.
State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
Front Microbiol. 2024 Sep 30;15:1437803. doi: 10.3389/fmicb.2024.1437803. eCollection 2024.
effectively colonizing the gut, secretes antimicrobial compounds and strengthens immune system function. Considering these health benefits, increasing its stress assessments efficiency could improve its commercial viability.
In this work, the resistance of FP41 to acid, bile salts, and freeze-drying was examined.
The findings showed that strain FP41 demonstrated a strong resistance to acid/bile salt stresses. The transcriptome revealed a significant up-regulation of various stress response genes, including those related to membrane integrity, glutamine metabolism, OsmC family protein, ABC transporters, and chaperonin. Subsequent research demonstrated that overexpression of three stress response-specific proteins, including glutamate decarboxylase GatD, osmotically induced bacterial protein OsmC, and membrane protein component CsbD, significantly increased the survival rate of Z204 under acid/bile salts stress. Notably, overexpression of the OsmC, CsbD, and GatD proteins also enhanced the survival of after freeze-drying.
The development of a unique cross-protection method is highlighted in this study, that might significantly increase cellular resistance to acid, bile salts, and cold stresses. This finding could significantly impact the way that is employed in industrial manufacturing processes.
有效定殖于肠道，分泌抗菌化合物并增强免疫系统功能。考虑到这些健康益处，提高其应激评估效率可提高其商业可行性。
在本研究中，检测了FP41对酸、胆盐和冻干的抗性。
研究结果表明，菌株FP41对酸/胆盐胁迫具有较强的抗性。转录组显示各种应激反应基因显著上调，包括与膜完整性、谷氨酰胺代谢、OsmC家族蛋白、ABC转运蛋白和伴侣蛋白相关的基因。后续研究表明，三种应激反应特异性蛋白的过表达，包括谷氨酸脱羧酶GatD、渗透诱导细菌蛋白OsmC和膜蛋白组分CsbD，显著提高了Z204在酸/胆盐胁迫下的存活率。值得注意的是，OsmC、CsbD和GatD蛋白的过表达也提高了冻干后的存活率。
本研究强调了一种独特的交叉保护方法的开发，这可能会显著提高细胞对酸、胆盐和冷胁迫的抗性。这一发现可能会对其在工业制造过程中的应用方式产生重大影响。
