Liu Ling, Helal Shimaa Elsayed, Peng Nan
National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
CABIO Biotech (Wuhan) Co. Ltd., Wuhan, China.
Biodes Res. 2023 Sep 29;5:0017. doi: 10.34133/bdr.0017. eCollection 2023.
Probiotics are the treasure of the microbiology fields. They have been widely used in the food industry, clinical treatment, and other fields. The equivocal health-promoting effects and the unknown action mechanism were the largest obstacles for further probiotic's developed applications. In recent years, various genome editing techniques have been developed and applied to explore the mechanisms and functional modifications of probiotics. As important genome editing tools, CRISPR-Cas systems that have opened new improvements in genome editing dedicated to probiotics. The high efficiency, flexibility, and specificity are the advantages of using CRISPR-Cas systems. Here, we summarize the classification and distribution of CRISPR-Cas systems in probiotics, as well as the editing tools developed on the basis of them. Then, we discuss the genome editing of probiotics based on CRISPR-Cas systems and the applications of the engineered probiotics through CRISPR-Cas systems. Finally, we proposed a design route for CRISPR systems that related to the genetically engineered probiotics.
益生菌是微生物学领域的瑰宝。它们已广泛应用于食品工业、临床治疗等领域。益生菌促进健康的效果尚不明确以及作用机制未知，是其进一步开发应用的最大障碍。近年来，各种基因组编辑技术已被开发并应用于探索益生菌的作用机制和功能修饰。作为重要的基因组编辑工具，CRISPR-Cas系统为益生菌的基因组编辑带来了新的进展。高效、灵活和特异性是使用CRISPR-Cas系统的优势。在此，我们总结了CRISPR-Cas系统在益生菌中的分类和分布，以及基于它们开发的编辑工具。然后，我们讨论基于CRISPR-Cas系统的益生菌基因组编辑以及通过CRISPR-Cas系统对工程益生菌的应用。最后，我们提出了一条与基因工程益生菌相关的CRISPR系统设计路线。
