Jo Yongseok, Song Kyungchul, Heo Seok-Jae, Suh Junghwan, Chae Hyun Wook, Rim John Hoon, Park Yongjung, Lim Jong Baek, Kim Ho-Seong, Kim Jeong-Ho
Department of Laboratory Medicine, Yongin Severance Hospital, Yonsei University College of Medicine, Yongin, South Korea.
Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea.
Heliyon. 2024 Sep 21;10(19):e38245. doi: 10.1016/j.heliyon.2024.e38245. eCollection 2024 Oct 15.
Appropriate continuous reference intervals (RIs) for serum insulin-like growth factor 1 (IGF-1) and insulin-like growth factor-binding protein 3 (IGFBP-3) are important for diagnosing growth hormone deficiency or excess.
We retrospectively reviewed serum IGF-1 and IGFBP-3 levels in Korean children aged 0-17 years who were diagnosed as healthy during a short stature workup in the outpatient clinics of three hospitals. IGF-1 and IGFBP-3 levels were measured using various immunoassays, including Liaison XL for IGF-1, an immunoradiometric assay (IRMA) for IGFBP-3 (n = 5522), and Immulite 2000 (n = 3036) and cobas e801 (n = 314). We established RIs from the 2.5th to 97.5th percentile RI curves using the lambda-mu-sigma (LMS) method for each sex group.
Pediatric serum continuous IGF-1 and IGFBP-3 reference percentiles by LMS method were found to be immunoassay method-dependent, but aligned relatively well with the manufacturers' RIs. IGFBP-3 levels displayed notable discrepancies among the different immunoassay methods.
Age- and sex-specific pediatric LMS based continuous reference intervals are method dependent and they should be calculated for dynamic parameters that show variations throughout childhood.
血清胰岛素样生长因子1（IGF-1）和胰岛素样生长因子结合蛋白3（IGFBP-3）合适的连续参考区间对于诊断生长激素缺乏或过量很重要。
我们回顾性分析了在三家医院门诊进行身材矮小检查时被诊断为健康的0至17岁韩国儿童的血清IGF-1和IGFBP-3水平。使用多种免疫测定法测量IGF-1和IGFBP-3水平，包括用于IGF-1的Liaison XL、用于IGFBP-3的免疫放射分析（IRMA）（n = 5522）、Immulite 2000（n = 3036）和cobas e801（n = 314）。我们使用lambda-mu-sigma（LMS）方法为每个性别组从第2.5百分位数到第97.5百分位数的参考区间曲线建立参考区间。
发现采用LMS方法的儿科血清连续IGF-1和IGFBP-3参考百分位数依赖于免疫测定方法，但与制造商的参考区间相对吻合。不同免疫测定方法之间的IGFBP-3水平存在显著差异。
基于年龄和性别的儿科LMS连续参考区间依赖于方法，对于在儿童期显示出变化的动态参数应进行计算。
