Department of Anesthesiology, Affiliated Jinhua Hospital, School of Medicine, Zhejiang University, Jinhua Municipal Central Hospital, Jinhua, China.
Luminescence. 2024 Oct;39(10):e4933. doi: 10.1002/bio.4933.
In this work, a fluorescent probe TPB-Cys derived from triphenylamine-benzofuranone was developed for monitoring the cysteine (Cys) level and imaging in living pulmonary cells under hypercapnia condition. A systematic hypoxia module was tried to cover both the in-solution test and pulmonary cellular imaging. The hypoxia condition did not obviously affect the fluorescence response signal during the in-solution test. The fluorescence signal at 540 enhanced in a dose-dependent enhancement along with the increase of the Cys concentration. The probe showed the advantages including relatively long linear range, high sensitivity, high stability, and high selectivity. With low cyto-toxicity, TPB-Cys achieved the monitoring of the endogenous Cys level in living pulmonary cells. Furthermore, it also realized the visualization of the affection of the hypoxia and hypoxia recovered conditions. This work was informative for both the accurate diagnosis and potential medical techniques.
在这项工作中，开发了一种基于三苯胺-苯并呋喃酮的荧光探针 TPB-Cys，用于监测高碳酸血症条件下活肺细胞中的半胱氨酸（Cys）水平并进行成像。尝试了一个系统的低氧模块来覆盖溶液内测试和肺细胞成像。在溶液内测试过程中，低氧条件并没有明显影响荧光响应信号。荧光信号在 540nm 处随着 Cys 浓度的增加呈剂量依赖性增强。该探针具有相对较长的线性范围、高灵敏度、高稳定性和高选择性等优点。TPB-Cys 具有低细胞毒性，可实现活肺细胞内内源性 Cys 水平的监测。此外，它还实现了对缺氧和缺氧恢复条件影响的可视化。这项工作对准确诊断和潜在的医学技术都具有重要意义。
