Anesthesiology Department, People's Hospital of Wucheng District, Jinhua, China.
Jinhua Advanced Research Institute, Jinhua, China.
Luminescence. 2024 Aug;39(8):e4854. doi: 10.1002/bio.4854.
In this work, a benzofuranone-derived fluorescent probe BFSF was developed for imaging the sulphite level in living hypoxia pulmonary cells. Under the excitation of 510 nm, BFSF showed a strong fluorescence response at 570 nm when reacted with sulphite. In the solution system, the constructed hypercapnia and serious hypercapnia conditions did not affect the fluorescence response. In comparison with the recently reported probes, BFSF suggested the advantages including rapid response, steady signal reporting, high specificity and low cytotoxicity upon living lung cells. Under a normal incubation atmosphere, BFSF realized the imaging of both exogenous and endogenous sulphite in living pulmonary cells. In particular, BFSF achieved imaging the decrease of the sulphite level under severe hypoxia as well as the recovery of the sulphite level with urgent oxygen supplement. With the imaging capability for the sulphite level in living pulmonary cells under hypoxia conditions, BFSF together with the information herein was meaningful for investigating the anaesthesia-related biological indexes.
在这项工作中，开发了一种基于苯并呋喃酮的荧光探针 BFSF，用于成像活缺氧肺细胞中的亚硫酸盐水平。在 510nm 的激发下，BFSF 与亚硫酸盐反应时在 570nm 处显示出强荧光响应。在溶液体系中，构建的高碳酸血症和严重高碳酸血症条件不会影响荧光响应。与最近报道的探针相比，BFSF 具有快速响应、稳定的信号报告、高特异性和低细胞毒性等优点，可用于活肺细胞。在正常孵育气氛下，BFSF 实现了活肺细胞中外源和内源性亚硫酸盐的成像。特别是，BFSF 实现了在严重缺氧下亚硫酸盐水平降低以及在紧急供氧下亚硫酸盐水平恢复的成像。BFSF 具有在缺氧条件下对活肺细胞中亚硫酸盐水平进行成像的能力，结合本文中的信息，对于研究与麻醉相关的生物学指标具有重要意义。
