Department of Pharmacy, Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou 324000, China.
Jinhua Institute of Zhejiang University, Zhejiang University, Jinhua, 321002, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Sep 5;317:124463. doi: 10.1016/j.saa.2024.124463. Epub 2024 May 12.
In this work, a triphenylamine-benzofuran-derived fluorescent probe TBSF was developed for monitoring the sulfite level in Chinese medicinal materials and imaging in living cells. In the testing system, under 445 nm excitation, TBSF responded to sulfite steadily with a 540 nm fluorescence reporting signal. The testing system showed advantages including high sensitivity, rapid response, and high selectivity. In particular, TBSF achieved the sulfite detection in the water decoction of Chinese medicinal materials from both addition and excessive fumigation. It also realized the intracellular imaging of both exogenous and endogenous sulfite in living HepG2 cells. The imaging in water decoction-treated cells inferred the potential for the interdisciplinary detection.
在这项工作中，开发了一种基于三苯胺-苯并呋喃的荧光探针 TBSF，用于监测中药材中亚硫酸盐水平并进行活细胞成像。在测试体系中，TBSF 在 445nm 激发下，以 540nm 的荧光报告信号稳定响应亚硫酸盐。该测试系统具有高灵敏度、快速响应和高选择性等优点。特别是，TBSF 实现了对中药材水提液中外加和过度熏硫的亚硫酸盐检测，也实现了活 HepG2 细胞中外源和内源性亚硫酸盐的细胞内成像。处理后的细胞成像推断出了跨学科检测的潜力。
