Howanitz P J, Howanitz J H, Skrodzki C A, Woloszyn T
Am J Clin Pathol. 1986 Jan;85(1):37-42. doi: 10.1093/ajcp/85.1.37.
Many protein methods are used for estimation of tissue receptor concentration. The authors compared performance, analytic variability, and accuracy of six protein methods used in these calculations. They found the Lowry protein procedure standardized with bovine serum albumin (BSA), usually considered the reference method, to be the most imprecise and most time consuming method. When the BSA standards from the Lowry procedure were assayed with the other methods, results ranged from 74 to 141% of expected. For three other protein standards, reactivity among the six methods varied almost twofold. Comparison of Lowry protein concentrations in cytosols from 46 tumors biopsies with other methods indicated best agreement was with an automated turbidometric (TCA) or a Coomassie dyebinding procedure. Use of protein standardization for the two direct spectrophotometric procedures decreased overestimation of receptor protein concentrations. Because receptor concentration is the quotient of receptor quantity and protein concentrations, tissue receptor results are dependent in part on standardization and choice of protein method.
许多蛋白质方法用于估计组织受体浓度。作者比较了这些计算中使用的六种蛋白质方法的性能、分析变异性和准确性。他们发现，通常被视为参考方法的用牛血清白蛋白（BSA）标准化的Lowry蛋白质测定法是最不精确且最耗时的方法。当用其他方法检测Lowry测定法中的BSA标准品时，结果在预期值的74%至141%之间。对于其他三种蛋白质标准品，六种方法之间的反应性差异几乎达到两倍。将46份肿瘤活检组织胞质溶胶中的Lowry蛋白质浓度与其他方法进行比较表明，与自动比浊法（TCA）或考马斯亮蓝染色法的一致性最好。对两种直接分光光度法使用蛋白质标准化减少了受体蛋白质浓度的高估。由于受体浓度是受体数量与蛋白质浓度的商，组织受体结果部分取决于标准化和蛋白质方法的选择。
