Powell B L, De La Garza M, Clark G M, McGuire W L
Breast Cancer Res Treat. 1981;1(1):33-5. doi: 10.1007/BF01807889.
The assay of estrogen receptor (ER) in human breast cancer biopsies is usually excellent using the dextrancoated charcoal method. If the cytosol protein concentration is below 1 mg/ml, however, underestimation of ER content occurs presumably due to adsorption of ER to the charcoal. This problem can be minimized if 1 mg/ml of bovine serum albumin (BSA) is added to the charcoal solution prior to use.
