Laboratory of Pathological Anatomy and Immunohistochemistry, School of Dentistry, Federal University of Pará, Rua Augusto Corrêa, 01, Belém, Pará, 66075-110, Brazil.
Cell Culture Laboratory, School of Dentistry, Federal University of Pará, Rua Augusto Corrêa, 01, Belém, Pará, 66075-110, Brazil.
Sci Rep. 2024 Feb 15;14(1):3856. doi: 10.1038/s41598-024-54132-7.
The aim of this study was to verify whether the expression of cell proliferation and apoptosis markers in different types of unicystic ameloblastoma (UA) is associated with the location of neoplastic cells. Immunohistochemical study with a sample of 32 cases of UA, 11 cases of conventional ameloblastoma (CAM) and ten dental follicles (DF) cases was performed. Cell proliferation was assessed using Ki-67 status, and apoptosis by caspase-3 expression. Mural UA (MUA) showed a higher immunostaining of Ki-67 (p < 0.05) and a lower immunostaining of Caspase-3 (p < 0.05) compared with luminal and intraluminal subtypes of UA and CAM. The neoplastic cells of the MUA's cystic capsule showed a higher expression of Ki-67 protein (p < 0.0001) and a lower expression of Caspase-3 (p < 0.0001) compared with the lumen. DF showed lower Ki-67 and Caspase-3 immunostaining (p < 0.05) than neoplasms. The higher immunoexpression of Ki-67 and the lower immunoexpression of Caspase-3 in MUA, in the parenchyma cells within the cystic capsule, suggest an association between the biological behaviour and location of neoplastic cells in a tumour.
本研究旨在验证不同类型的单囊型成釉细胞瘤（UA）中细胞增殖和凋亡标志物的表达是否与肿瘤细胞的位置有关。对 32 例 UA、11 例常规成釉细胞瘤（CAM）和 10 例牙滤泡（DF）病例进行了免疫组织化学研究。Ki-67 状态评估细胞增殖，caspase-3 表达评估细胞凋亡。与 UA 和 CAM 的腔型和腔内型相比，壁型 UA（MUA）的 Ki-67 免疫染色更高（p<0.05），Caspase-3 免疫染色更低（p<0.05）。MUA 的囊腔胶囊中的肿瘤细胞 Ki-67 蛋白表达更高（p<0.0001），Caspase-3 表达更低（p<0.0001）。DF 的 Ki-67 和 Caspase-3 免疫染色均低于肿瘤（p<0.05）。MUA 中囊腔胶囊内实质细胞的 Ki-67 免疫表达较高，Caspase-3 免疫表达较低，提示肿瘤中肿瘤细胞的生物学行为和位置之间存在关联。
