Division of Genomic Resources, ICAR-NBAIR, Hebbal, Bengaluru, India, 560024; Department of Entomology, College of Agriculture, IGKV, Raipur, Chhattisgarh, India, 492012; School of Agriculture, Lovely Professional University, Phagwara, Punjab, India, 144411.
Division of Genomic Resources, ICAR-NBAIR, Hebbal, Bengaluru, India, 560024.
Chemosphere. 2024 Nov;367:143523. doi: 10.1016/j.chemosphere.2024.143523. Epub 2024 Oct 13.
The pink or hibiscus mealybug, Maconellicoccus hirsutus, is a serious pest of grapes, jute, and mesta, causing severe yield losses in India and other countries. Chemical control remains the foremost choice for farmers to manage this pest. As insecticides break down over time due to biotic and abiotic factors, insects are exposed to varying levels of these exogenous compounds. Several studies have reported that sublethal doses affect insect physiology, but only a few have examined the changes in gene expression at the molecular level. Therefore, the present study was conducted to elucidate the molecular mechanisms in M. hirsutus exposed to sublethal doses of buprofezin 25 SC. Life table analysis revealed increased fecundity in M. hirsutus exposed to the sublethal dose. A total of 1,744 differentially expressed genes were identified between the buprofezin-treated and untreated samples using transcriptome analysis. These genes were primarily associated with ribosomal proteins, proteases, cuticular proteins, and cytoskeletal structures. Ribosomes and phagosomes were the most highly enriched pathways. Interestingly, most of the DEGs were involved in restoring homeostasis rather than detoxification. To validate our RNA-sequencing results, qRT-PCR validation was performed on ten randomly selected genes. Overall, our findings provide valuable insights into intermittent changes in stress-coping genes, apart from detoxification genes.
桃蚜粉蚧，又称毛毡瘿绵蚜，是葡萄、黄麻和大麻的严重害虫，在印度和其他国家造成严重的产量损失。化学防治仍然是农民管理这种害虫的首要选择。由于生物和非生物因素，杀虫剂会随着时间的推移而分解，昆虫会接触到不同水平的这些外源性化合物。有几项研究报告称，亚致死剂量会影响昆虫的生理机能，但只有少数研究从分子水平上研究了基因表达的变化。因此，本研究旨在阐明桃蚜粉蚧暴露于吡丙醚 25 SC 亚致死剂量下的分子机制。生命表分析表明，暴露于亚致死剂量下的桃蚜粉蚧的繁殖力增加。使用转录组分析，在吡丙醚处理和未处理的样本之间鉴定出了 1744 个差异表达基因。这些基因主要与核糖体蛋白、蛋白酶、角质蛋白和细胞骨架结构有关。核糖体和吞噬体是最富集的途径。有趣的是，大多数差异表达基因参与的是恢复体内平衡，而不是解毒。为了验证我们的 RNA-seq 结果，我们对十个随机选择的基因进行了 qRT-PCR 验证。总的来说，我们的研究结果除了提供解毒基因外，还提供了关于应激应对基因间歇性变化的有价值的见解。
