State Key Laboratory of Heavy Oil Processing & College of Chemistry and Chemical Engineering, China University of Petroleum (East China), 66 West Changjiang Road, Qingdao, Shandong 266580, China.
State Key Laboratory of Heavy Oil Processing & College of Chemistry and Chemical Engineering, China University of Petroleum (East China), 66 West Changjiang Road, Qingdao, Shandong 266580, China.
Int J Biol Macromol. 2024 Nov;281(Pt 4):136482. doi: 10.1016/j.ijbiomac.2024.136482. Epub 2024 Oct 13.
Traditional hemostatic hydrogels face considerable limitations in achieving rapid control of severe bleedings, a crucial factor in reducing casualties in both military and civilian settings. This study presents a chitosan-based hemostatic hydrogel with interconnected secondary macropores designed to enhance interactions with blood clotting components by reducing diffusion resistance and increasing contact area. The macropores were created using a straightforward process involving NaOH-mediated SiO template dissolution and NH generation. The resulting macroporous structure increased the hydrogel's overall porosity without compromising its viscoelasticity. Functional studies demonstrated that the macroporous hydrogel effectively concentrated and adsorbed blood clotting components, while also facilitating the delivery of artificially embedded clotting factor to further expedite clot formation. These combined actions resulted in improve hemostatic efficacy, reducing whole blood clotting time by over 94 % in vitro. Furthermore, in vivo studies using rat tail amputation and liver injury models showed a reduction in blood loss by over 65 % and a decrease in bleeding time by over 70 %. Additionally, the porous chitosan hydrogel exhibited minimal biotoxicity and promoted biodegradability in vivo. In conclusion, this work introduces a macroporous chitosan-based hemostatic hydrogel with great potential for rapid hemorrhage control.
传统的止血水凝胶在实现对严重出血的快速控制方面面临着相当大的限制，而这是降低军事和民用环境中伤亡的关键因素。本研究提出了一种基于壳聚糖的具有互连通孔的二级宏观结构的止血水凝胶，旨在通过降低扩散阻力和增加接触面积来增强与血液凝固成分的相互作用。这些大孔是通过一种简单的方法制造的，涉及到 NaOH 介导的 SiO 模板溶解和 NH 的生成。所得到的大孔结构增加了水凝胶的整体孔隙率，同时保持了其粘弹性。功能研究表明，大孔水凝胶能够有效地浓缩和吸附血液凝固成分，同时促进人工嵌入的凝血因子的传递，进一步加速凝血形成。这些综合作用提高了止血效果，将体外全血凝固时间缩短了 94%以上。此外，使用大鼠尾巴切断和肝脏损伤模型的体内研究表明，出血量减少了 65%以上，出血时间减少了 70%以上。此外，多孔壳聚糖水凝胶表现出最小的生物毒性，并促进了体内的生物降解。总之，这项工作介绍了一种具有快速止血潜力的基于壳聚糖的大孔止血水凝胶。
