Fujian Provincial Key Laboratory of Polymer Materials, College of Material Science and Engineering, Fujian Normal University, Fujian 350007, China.
College of Life Science, Fujian Normal University, Fujian 350007, China.
Carbohydr Polym. 2020 Nov 1;247:116669. doi: 10.1016/j.carbpol.2020.116669. Epub 2020 Jun 24.
A quick control of heavy hemorrhaging is critical to save the life of injured person. Herein we developed a hemostat of chitosan@calcium alginate microspheres through combination of microemulsion, polyelectrolyte complexation coating, and thermally induced phase separation. The alginate coated microspheres featured a structure of a porous chitosan core and a compact calcium alginate shell layer. Their in vitro and in vivo hemostatic properties were evaluated by the whole blood clotting kinetics, and rat tail amputation and liver laceration models. Compared to the porous chitosan microspheres, the alginate coated microspheres showed much enhanced hemostatic efficiency. The latter formed bigger blood clots; its hemostatic time on the liver laceration was substantially shortened to 53 ± 10 s from 107 ± 9 s of the former. It was shown that calcium ions released from the calcium alginate layer may accelerate the formation of blood clots. Such a biocompatible microsphere is a promising quick hemostat for controlling traumatic bleeding.
快速控制大出血对于挽救伤员的生命至关重要。在此，我们通过微乳液、聚电解质复合涂层和热致相分离相结合的方法，开发了一种壳聚糖@海藻酸钠微球止血剂。海藻酸钠涂层微球具有多孔壳聚糖核和致密海藻酸钠壳层的结构。通过全血凝血动力学和大鼠尾切断和肝撕裂模型评估了它们的体外和体内止血性能。与多孔壳聚糖微球相比，海藻酸钠涂层微球显示出更高的止血效率。后者形成更大的血栓；其在肝撕裂伤中的止血时间从前者的 107 ± 9s 显著缩短至 53 ± 10s。研究表明，海藻酸钠层释放的钙离子可能会加速血栓的形成。这种生物相容性微球是一种有前途的快速止血剂，可用于控制创伤性出血。
