Department of Bionanotechnology, Graduate School, Hanyang University, Seoul, 04763, Korea.
Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea.
Sci Rep. 2024 Oct 15;14(1):24117. doi: 10.1038/s41598-024-75080-2.
Preeclampsia is a hypertensive disorder of pregnancy that can lead to stillbirth and preterm birth if not treated promptly. Currently, the diagnosis of preeclampsia relies on clinical symptoms such as hypertension and proteinuria, along with invasive blood tests. Here, we investigate the role of soluble proteins and exosomes in noninvasive diagnosing preeclampsia non-invasively using maternal urine and urine-derived exosomes. We quantified the levels of particles and the presence of TSG101 and CD63 in urine and urinary exosomes via the biologically intact exosome separation technology (BEST) platform. Then, we obtained higher levels of soluble proteins such as fms-like tyrosine kinase-1 (sFlt-1) and placental growth factor (PlGF) from urine as it was than urinary exosomes. Compared to commercial blood tests, the sensitivity of the sFlt-1/PlGF ratio was found to be 4.0 times higher in urine tests and 1.5 times higher in tests utilizing urine-derived exosomes. Our findings offer promising possibilities for the early and non-invasive identification of high-risk individuals at risk of preeclampsia, allowing for comprehensive preventive management.
子痫前期是一种妊娠高血压疾病，如果不及时治疗，可能导致死胎和早产。目前，子痫前期的诊断依赖于临床症状，如高血压和蛋白尿，以及侵入性血液检查。在这里，我们研究了使用母体尿液和尿液衍生的外泌体，通过可溶性蛋白和外泌体在非侵入性诊断子痫前期中的作用。我们通过生物完整外泌体分离技术（BEST）平台定量了尿液和尿外泌体中颗粒的水平和 TSG101 和 CD63 的存在。然后，我们从尿液中获得了更高水平的可溶性蛋白，如血管内皮生长因子受体 1（sFlt-1）和胎盘生长因子（PlGF）。与商业血液检查相比，尿液中 sFlt-1/PlGF 比值的灵敏度比血液检查高 4.0 倍，尿液衍生外泌体的灵敏度比血液检查高 1.5 倍。我们的研究结果为早期和非侵入性识别高危人群中可能患有子痫前期的个体提供了有希望的可能性，从而可以进行全面的预防管理。
