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环状 RNA CDR1as/miR-214-3p 调控轴在 Legg-Calvé-Perthes 病中的鉴定。

Identification of circRNA CDR1as/miR-214-3p regulatory axis in Legg-Calvé-Perthes disease.

机构信息

Orthopedic Hospital, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, No. 1519, Dongyue Avenue, Nanchang, Jiangxi Province, 330006, P.R. China.

出版信息

Orphanet J Rare Dis. 2024 Oct 15;19(1):380. doi: 10.1186/s13023-024-03394-5.

DOI:10.1186/s13023-024-03394-5

PMID:39407304

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11481470/

Abstract

BACKGROUND

Legg-Calvé-Perthes disease (LCPD) commonly occurs among adolescents, threatening their health. However, the potential mechanism underlying LCPD remains unclear. miR-214-3p is shown as a critical role in LCPD development with unspecified upstream regulators.

METHODS

Levels of miR-214-3p and circCDR1as in healthy controls and LCPD patients were determined by qRT-PCR. The role of circCDR1as/miR-214-3p axis in LCPD was determined by testing the cell viability and apoptosis in TC28 cells and primary chondrocytes. Regulation between circCDR1as and miR-214-3p was examined by RIP and ChIP assays. The inflammatory response and angiogenesis were evaluated by M2 macrophage polarization and HUVECs tumor formation.

RESULTS

circCDR1as was overexpressed in LCPD patients with a negative correlation with miR-214-3p. Inhibition of circCDR1as alleviated the cell viability and apoptosis of DEX-treated chondrocytes, stimulated M2 macrophage polarization and angiogenesis. miR-214-3p was proved as a downstream effector to participate in circCDR1as mediated actions. circCDR1as recruited PRC2 complex to epigenetically suppress miR-214-3p.

CONCLUSION

Our study illustrated the role and mechanism of circCDR1as in LCPD development by targeting miR-214-3p, highlighting its potential in the therapy for LCPD.

摘要

背景

Legg-Calvé-Perthes 病（LCPD）常见于青少年，威胁其健康。然而，LCPD 的潜在机制尚不清楚。miR-214-3p 在 LCPD 发展中起着关键作用，但上游调节因子尚不清楚。

方法

通过 qRT-PCR 测定健康对照组和 LCPD 患者中 miR-214-3p 和 circCDR1as 的水平。通过检测 TC28 细胞和原代软骨细胞的细胞活力和凋亡来确定 circCDR1as/miR-214-3p 轴在 LCPD 中的作用。通过 RIP 和 ChIP 测定来检查 circCDR1as 和 miR-214-3p 之间的调节关系。通过 M2 巨噬细胞极化和 HUVECs 肿瘤形成评估炎症反应和血管生成。

结果

circCDR1as 在 LCPD 患者中表达上调，与 miR-214-3p 呈负相关。抑制 circCDR1as 可减轻 DEX 处理的软骨细胞的细胞活力和凋亡，刺激 M2 巨噬细胞极化和血管生成。miR-214-3p 被证明是参与 circCDR1as 介导作用的下游效应物。circCDR1as 募集 PRC2 复合物来表观遗传抑制 miR-214-3p。