School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China.
School of Pharmacy, Jilin Medical University, Jilin 132013, China.
Molecules. 2024 Sep 24;29(19):4530. doi: 10.3390/molecules29194530.
This study describes the purification and preparation of tricin (5, 7, 4-trihydroxy-3, 5-dimethoxyflavone) from Carex Meyeriana Kunth via adsorption and desorption using macroporous resins and high-performance liquid chromatography. Six resins were tested to evaluate the static adsorption and desorption capacities. The HPD-300 resin was selected as the adsorption material to enrich tricin because of its suitable adsorption and desorption capacities. Adsorption thermodynamics and kinetics were studied on HPD-300 resin, and the results agreed with the Langmuir model and quasi-second-order kinetics model, respectively. The parameters of the dynamic adsorption and desorption tests were then optimized. The purity of tricin increased from 2.6 mg/g to 45.1 mg/g with a recovery yield of 76.4% after purification using HPD-300 resin. Then, Prep-HPLC was used to further purify tricin. The purity of tricin reached 99.4%, with a recovery yield of 78.0% thereafter. Tricin exerts an inhibitory effect on the proliferation of various tumor cells, including gastric cancer SGC-7901 cells. It significantly suppresses cell colony formation while also altering cell cycle progression metabolism by decreasing the proportion of cells in the G/G phase and increasing the proportion in the S and G/M phases. Additionally, tricin affects the efficiency of SGC-7901 cell lactate production, ATP content, and glucose uptake. These findings suggest that tricin may impede tumor cell proliferation through its impact on cell cycle progression and energy metabolism.
本研究描述了通过吸附和解吸使用大孔树脂和高效液相色谱法从薹草中提取和制备木樨草素（5,7,4-三羟基-3,5-二甲氧基黄酮）。测试了六种树脂以评估静态吸附和解吸能力。选择 HPD-300 树脂作为吸附材料来富集木樨草素，因为它具有合适的吸附和解吸能力。研究了 HPD-300 树脂的吸附热力学和动力学，结果分别符合朗缪尔模型和拟二级动力学模型。然后优化了动态吸附和解吸试验的参数。经过 HPD-300 树脂纯化后，木樨草素的纯度从 2.6mg/g 增加到 45.1mg/g，回收率为 76.4%。然后，使用 Prep-HPLC 进一步纯化木樨草素。此后，木樨草素的纯度达到 99.4%，回收率为 78.0%。木樨草素对包括胃癌 SGC-7901 细胞在内的各种肿瘤细胞的增殖具有抑制作用。它显著抑制细胞集落形成，同时通过降低 G/G 期细胞的比例和增加 S 和 G/M 期细胞的比例来改变细胞周期进程代谢。此外，木樨草素影响 SGC-7901 细胞乳酸产生、ATP 含量和葡萄糖摄取的效率。这些发现表明，木樨草素可能通过影响细胞周期进程和能量代谢来抑制肿瘤细胞增殖。
