CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Molecules. 2020 Jul 31;25(15):3511. doi: 10.3390/molecules25153511.
Flavonoids are the main constituents of Goji berries and have good biological and pharmacological activities. The mixed-mode macroporous adsorption resins (MARs) for purification of flavonoids from Goji berries through computer-assisted calculation of the molecular size of flavonoids and the precise matching of MAR physical and chemical properties was firstly developed in the present study. Ten varieties of MARs with suitable molecular dimensions and polarities were used for investigating the adsorption/desorption behaviors of the flavonoids. Both AUKJ-1 and BWKX-1 showed higher separation efficiency than other MARs and then were mixed in different ratios to constitute a mixed-mode macroporous adsorption resin to obtain the optimal adsorption phase. Under optimal conditions, total flavonoid content of purified flavonoid (p-FLA) extract increased from 0.97% to 36.88% after one purification. The p-FLA extract from Goji berries significantly improved the expression of six genes with anti-aging effects and played an important role in aging-related Alzheimer's disease by down-regulating Aβ expression.
类黄酮是枸杞的主要成分，具有良好的生物学和药理学活性。本研究首次通过计算机计算类黄酮的分子大小和精确匹配 MAR 物理化学性质，开发了用于从枸杞中纯化类黄酮的混合模式大孔吸附树脂 (MAR)。使用十种具有合适分子尺寸和极性的 MAR 来研究类黄酮的吸附/解吸行为。AUKJ-1 和 BWKX-1 均表现出比其他 MAR 更高的分离效率，然后以不同比例混合以构成混合模式大孔吸附树脂，以获得最佳吸附相。在最佳条件下，经一次纯化后，纯化的类黄酮（p-FLA）提取物中的总黄酮含量从 0.97%增加到 36.88%。枸杞 p-FLA 提取物显著提高了具有抗衰老作用的六个基因的表达水平，并通过下调 Aβ 表达在与衰老相关的阿尔茨海默病中发挥重要作用。
