College of Pharmacy, Yanbian University, Yanji 133000, China.
School of Pharmacy and Medicine, Tonghua Normal University, Tonghua 134002, China.
Molecules. 2024 Sep 27;29(19):4602. doi: 10.3390/molecules29194602.
Turcz., a botanical species with a rich historical background in traditional medicine for detoxification and insecticidal applications, possesses a vast, yet largely unexplored, therapeutic potential. This study primarily focused on conducting a qualitative phytochemical analysis of the plant, determining the active ingredient content and antioxidant activity of various solvent extracts. The qualitative phytochemical analysis revealed the presence of 12 different types of phytochemicals within the plant. Utilizing ultraviolet-visible spectrophotometry, we identified 11 active ingredients in 4 solvent extracts. Notably, the methanol extract was found to contain high concentrations of total carbohydrate, total monoterpenoid, total phenolic, total tannin, and total triterpenoid. In the antioxidant experiment, the methanol extract demonstrated superior scavenging abilities against 1,1-diphenyl-2-picrylhydrazyl radical, 2,2'-azino-(3-ethylbenzothiazoline-6-sulfonicacid) diammonium salt, superoxide anion radical, and hydrogen peroxide, outperforming other extracts in chelation experiments aimed at reducing iron and metal ions. Consequently, the methanol extract was selected for further investigation. Subsequent ultrahigh-performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry analysis revealed that the methanol extract contained 39 compounds, primarily phenolic compounds and triterpenoid saponins. Three stability assessments confirmed the extract's stability under high temperatures, varying pH levels, and simulated gastrointestinal processes. Additionally, oil stability testing demonstrated its antioxidant capacity in extra virgin olive oil and cold-pressed sunflower seed oil media. An oral acute toxicity experiment conducted on mice not only confirmed the absence of acute toxicity in the methanol extract but also provided a dose reference for subsequent gastric protection experiments. Notably, the methanol extract exhibited significant gastroprotective effects against ethanol-induced gastric lesions in rats, as evidenced by histopathological and biochemical analyses. Specifically, the extract reduced levels of malondialdehyde, alanine aminotransferase, and aspartate aminotransferase while increasing glutathione, nitric oxide, and catalase, indicating its gastroprotective mechanism. These findings suggest that the methanol extract from the aerial part of could be a promising therapeutic agent for conditions associated with oxidative imbalances. They underscore the plant's potential therapeutic benefits and offer valuable insights into its antioxidant properties, thereby broadening our understanding of its medicinal potential.
Turcz.，一种在传统医学中具有丰富解毒和杀虫应用历史背景的植物物种，具有广泛但尚未充分开发的治疗潜力。本研究主要集中于对植物进行定性植物化学分析，确定各种溶剂提取物的活性成分含量和抗氧化活性。定性植物化学分析表明，植物中存在 12 种不同类型的植物化学物质。利用紫外可见分光光度法，我们在 4 种溶剂提取物中鉴定出 11 种活性成分。值得注意的是，甲醇提取物中总碳水化合物、总单萜、总酚、总单宁和总三萜的含量较高。在抗氧化实验中，甲醇提取物对 1,1-二苯基-2-苦基肼自由基、2,2'-联氮基-（3-乙基苯并噻唑啉-6-磺酸）二铵盐、超氧阴离子自由基和过氧化氢的清除能力表现出优越性，在螯合实验中也优于其他提取物，可用于还原铁和金属离子。因此，选择甲醇提取物进行进一步研究。随后的超高效液相色谱-电喷雾电离-四极杆飞行时间质谱分析显示，甲醇提取物中含有 39 种化合物，主要为酚类化合物和三萜皂苷。三种稳定性评估证实了提取物在高温、不同 pH 值和模拟胃肠道过程下的稳定性。此外，油稳定性测试表明，它在特级初榨橄榄油和冷榨葵花籽油介质中具有抗氧化能力。对小鼠进行的口服急性毒性实验不仅证实了甲醇提取物无急性毒性，还为后续胃保护实验提供了剂量参考。值得注意的是，甲醇提取物对乙醇诱导的大鼠胃损伤具有显著的胃保护作用，这可以通过组织病理学和生化分析得到证明。具体来说，提取物降低了丙二醛、丙氨酸氨基转移酶和天冬氨酸氨基转移酶的水平，同时增加了谷胱甘肽、一氧化氮和过氧化氢酶的水平，表明其胃保护机制。这些发现表明，来自 Turcz. 的地上部分的甲醇提取物可能是治疗与氧化失衡相关疾病的有前途的治疗剂。它们强调了该植物的潜在治疗益处，并提供了对其抗氧化特性的宝贵见解，从而拓宽了我们对其药用潜力的理解。
