Department of Molecular and Cellular Biology, Faculty of Nature and Life Sciences, University of Abbes Laghrour, Khenchela 40000, Algeria.
Laboratory of Biotechnology, Water, Environment and Health (LBWEH), University of Abbes Laghrour, Khenchela 40000, Algeria.
Molecules. 2024 Sep 28;29(19):4614. doi: 10.3390/molecules29194614.
Infectious diseases, including vector-borne and antibiotic-resistant infections, present significant global health challenges, necessitating the exploration of natural alternatives for disease control. In this study, we investigated the essential oil (EO) profile as well as larvicidal and antibacterial properties of two wild Apiaceae species used in Algeria: L. (DCEO) and Mill. (FVEO). EO was extracted from the aerial parts by steam distillation and analyzed using Gas Chromatography Mass Spectrometry (GC/MS). Major constituents identified in DCEO were geranyl acetate (50.07%) and elemicin (10.77%), while FVEO contained estragole (24.93%), fenchone (20.20%), and α-phellandrene (17.96%). Both EOs were highly effective towards larvae, with low LC values of 30.6 ± 1.06 ppm for DCEO and 34.7 ± 1.06 ppm for FVEO, indicating their potential as bioinsecticides due to their bioactivity and bioavailability. Additionally, the two Eos demonstrated moderate antibacterial efficacy against gram-positive bacteria, ATCC 25923 and MRSA ATCC 43300, respectively, with DCEO showing MIC values of 10 and 20 mg/mL, respectively, and FVEO exhibiting MIC values > 20 mg/mL. However, both EOs showed limited effectiveness against gram-negative bacteria, ATCC 25922 and ATCC 700603. These results highlight the potential applications of DCEO and FVEO as natural bioinsecticides and antibacterial agents, offering promising avenues for further research and development in pest control and food preservation.
传染病，包括媒介传播和抗生素耐药感染，对全球健康构成重大挑战，需要探索用于疾病控制的天然替代品。在这项研究中，我们研究了两种在阿尔及利亚使用的野生伞形科植物的精油（EO）特征以及杀幼虫和抗菌特性：L.（DCEO）和M ill.（FVEO）。通过蒸汽蒸馏从地上部分提取精油，并使用气相色谱质谱联用仪（GC/MS）进行分析。DCEO 中的主要成分是乙酸香叶酯（50.07％）和 elemicin（10.77％），而 FVEO 则含有 estragole（24.93％），fenchone（20.20％）和α-蒎烯（17.96％）。两种精油对幼虫均具有高度有效性，DCEO 的 LC 值为 30.6±1.06ppm，FVEO 的 LC 值为 34.7±1.06ppm，表明由于其生物活性和生物利用度，它们具有作为生物杀虫剂的潜力。此外，两种 EO 对革兰氏阳性菌，ATCC 25923 和 MRSA ATCC 43300 均具有适度的抗菌功效，DCEO 的 MIC 值分别为 10 和 20mg/mL，FVEO 的 MIC 值>20mg/mL。但是，两种 EO 对革兰氏阴性菌，ATCC 25922 和 ATCC 700603 的效果有限。这些结果突出了 DCEO 和 FVEO 作为天然生物杀虫剂和抗菌剂的潜在应用，为害虫控制和食品保存的进一步研究和开发提供了有希望的途径。
