Department of Chemistry, Technology, and Biotechnology of Food, Faculty of Chemistry, Gdańsk University of Technology, 11/12 G.Narutowicza Str., 80-233 Gdansk, Poland.
Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdańsk University of Technology, 11/12 G.Narutowicza Str., 80-233 Gdansk, Poland.
Int J Mol Sci. 2022 May 14;23(10):5505. doi: 10.3390/ijms23105505.
The article presents a new approach in the purification of chitosan (CS) hydrogel in order to remove a significant amount of endotoxins without changing its molecular weight and viscosity. Two variants of the method used to purify CS hydrogels from endotoxins were investigated using the PyroGene rFC Enzymatic Cascade assay kit. The effect of the CS purification method was assessed in terms of changes in the dynamic viscosity of its hydrogels, the molecular weight of the polymer, microbiological purity after refrigerated storage and cytotoxicity against L929 cells based on the ISO 10993-5:2009(E) standard. The proposed purification method 1 (M1) allows for the removal of significant amounts of endotoxins: 87.9-97.6% in relation to their initial concentration in the CS hydrogel without affecting the solution viscosity. Moreover, the final solutions were sterile and microbiologically stable during storage. The M1 purification method did not change the morphology of the L929 cells.
本文提出了一种新的壳聚糖（CS）水凝胶纯化方法，旨在去除大量内毒素，同时保持其分子量和粘度不变。使用 PyroGene rFC 酶级联检测试剂盒，研究了两种用于从内毒素中纯化 CS 水凝胶的方法变体。根据 ISO 10993-5:2009(E)标准，从水凝胶的动态粘度、聚合物分子量、冷藏储存后的微生物纯度和对 L929 细胞的细胞毒性等方面评估了 CS 水凝胶的纯化方法的效果。所提出的纯化方法 1（M1）能够去除大量的内毒素：与 CS 水凝胶中内毒素的初始浓度相比，去除率为 87.9-97.6%，而不会影响溶液的粘度。此外，最终溶液在储存过程中无菌且微生物稳定。M1 纯化方法未改变 L929 细胞的形态。
