Institute of Nanoscience & Nanotechnology, NCSR "Demokritos", 15341 Aghia Paraskevi, Greece.
Immunoassays/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR "Demokritos", 15341 Aghia Paraskevi, Greece.
Molecules. 2024 Oct 7;29(19):4736. doi: 10.3390/molecules29194736.
The enhanced and direct immobilization of the enzyme horseradish peroxidase on poly(methyl methacrylate) (PMMA) microchannel surfaces to create a miniaturized enzymatic reactor for the biocatalytic oxidation of phenols is demonstrated. Enzyme immobilization occurs by physical adsorption after oxygen plasma treatment, which micro-nanotextures the PMMA surfaces. A five-fold enhancement in immobilized enzyme activity was observed, attributed to the increased surface area and, therefore, to a higher quantity of immobilized enzymes compared to an untreated PMMA surface. The enzymatic reaction yield reached 75% using a flow rate of 2.0 μL/min for the reaction mixture. Additionally, the developed microreactor was reused more than 16 times without affecting the enzymatic conversion yield. These results demonstrate the potential of microchannels with plasma micro/nanotextured surfaces for the rapid and facile fabrication of microfluidic enzymatic microreactors with enhanced catalytic activity and stability.
通过氧等离子体处理后物理吸附实现辣根过氧化物酶在聚甲基丙烯酸甲酯（PMMA）微通道表面的增强和直接固定，从而构建用于苯酚生物催化氧化的微型酶反应器。这种方法使 PMMA 表面微观纳纹化，从而实现酶的固定化。与未经处理的 PMMA 表面相比，固定化酶的活性提高了五倍，这归因于比表面积的增加，进而固定化酶的数量也有所增加。在 2.0 μL/min 的流速下，反应混合物的酶反应产率达到 75%。此外，所开发的微反应器在不影响酶转化产率的情况下可以重复使用 16 次以上。这些结果表明，具有等离子体微观纳纹表面的微通道具有用于快速简便地制造具有增强催化活性和稳定性的微流控酶微反应器的潜力。
