Irie Koichiro, Sato Satsuki, Kamata Yohei, Mochida Yuki, Hirata Takahisa, Komaki Motohiro, Yamamoto Tatsuo
Department of Preventive Dentistry and Dental Public Health, Kanagawa Dental University, Yokosuka 238-8580, Kanagawa, Japan.
Department of Periodontology, Kanagawa Dental University, Yokosuka 238-8580, Kanagawa, Japan.
J Clin Med. 2024 Oct 3;13(19):5904. doi: 10.3390/jcm13195904.
Salivary lactate dehydrogenase (LD) levels are a feasible and useful parameter for screening periodontal diseases. The periodontal inflamed surface area (PISA) is useful to clinically assess periodontal diseases. However, PISA is difficult to calculate and PISA-compatible screening kits are required. We aimed to investigate the association between salivary LD levels, using a test kit, and PISA and PISA-Japanese and determine the feasibility and reliability of the salivary LD test kit for evaluation of periodontal status. This study included 110 patients (66.4% female, median and 25-75 percentiles of age were 66.5 and 53.0-75.0 years, respectively) who visited the Dental University Clinic in Japan. Resting saliva samples were collected from each participant and LD levels were evaluated in real time using a kit featuring an integer scale ranging from 1 to 10. PISA and PISA-Japanese were calculated using periodontal parameters. The median salivary LD level was 4.0. The medians of PISA and PISA-Japanese were 46.9 and 61.0, respectively. Salivary LD levels were positively correlated with the bleeding on probing rate ( = 0.626, < 0.001), PISA (r = 0.560, < 0.001), and PISA-Japanese ( = 0.581, < 0.001). Our results suggest that salivary LD levels assessed using the salivary LD kit showed a significantly positive correlation with PISA and PISA-Japanese. In addition, we developed the PISA estimation formula using salivary LD levels measured with a test kit, sex, and age.
唾液乳酸脱氢酶（LD）水平是筛查牙周疾病的一个可行且有用的参数。牙周炎症表面积（PISA）有助于临床评估牙周疾病。然而，PISA难以计算，需要与PISA兼容的筛查试剂盒。我们旨在研究使用检测试剂盒测得的唾液LD水平与PISA及日本版PISA之间的关联，并确定唾液LD检测试剂盒评估牙周状况的可行性和可靠性。本研究纳入了110名就诊于日本牙科大学诊所的患者（女性占66.4%，年龄中位数以及第25至75百分位数分别为66.5岁和53.0 - 75.0岁）。从每位参与者收集静息唾液样本，并使用一个刻度范围为1至10的整数量表的试剂盒实时评估LD水平。使用牙周参数计算PISA和日本版PISA。唾液LD水平中位数为4.0。PISA和日本版PISA的中位数分别为46.9和61.0。唾液LD水平与探诊出血率（r = 0.626，P < 0.001）、PISA（r = 0.560，P < 0.001）和日本版PISA（r = 0.581，P < 0.001）呈正相关。我们的结果表明，使用唾液LD试剂盒评估的唾液LD水平与PISA和日本版PISA呈显著正相关。此外，我们利用试剂盒测得的唾液LD水平、性别和年龄开发了PISA估计公式。
