Klimov Dmitry I, Zharikov Alexey A, Zezina Elena A, Kotenkova Elena A, Zaiko Elena V, Bataeva Dagmara S, Semenova Anastasia A, Yushina Yulia K, Yaroslavov Aleksander A, Zezin Alexey A
Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie gory 1-3, Moscow 119991, Russia.
Enikolopov Institute of Synthetic Polymeric Materials, Russian Academy of Sciences, Profsoyuznaya st. 70, Moscow 117393, Russia.
Polymers (Basel). 2024 Oct 8;16(19):2842. doi: 10.3390/polym16192842.
In order to control pathogenic microorganisms, three polymer compositions were prepared and tested. First, a water-soluble positively charged polycomplex was synthesized via the electrostatic binding of anionic polyacrylic acid to an excess of polyethylenimine to enhance the biocidal activity of the polycation. Second, an aqueous solution of AgNO was added to the polycomplex, thus forming a ternary polycation-polyanion-Ag complex with an additional antimicrobial effect. Third, the resulting ternary complex was subjected to UV irradiation, which ensured the conversion of Ag ions into Ag nanoparticles ranging in size mainly from 10 to 20 nm. Aqueous solutions of the polymer compositions were added to suspensions of the Gram-positive bacteria and the Gram-negative bacteria , with the following main results: (a) Upon the addition of the binary polycomplex, 30% or more of the cells survived after 20 h. (b) The ternary complex killed bacteria but was ineffective against bacteria. (c) When the ternary complex with Ag nanoparticles was added, the percentage of surviving cells of both types did not exceed 0.03%. The obtained results are valuable for the development of antibacterial formulations.
为了控制病原微生物，制备并测试了三种聚合物组合物。首先，通过阴离子聚丙烯酸与过量聚乙烯亚胺的静电结合合成了一种水溶性带正电荷的聚络合物，以增强聚阳离子的杀菌活性。其次，将硝酸银水溶液加入到聚络合物中，从而形成具有额外抗菌作用的三元聚阳离子 - 聚阴离子 - 银络合物。第三，对所得的三元络合物进行紫外线照射，这确保了银离子转化为尺寸主要在10至20纳米范围内的银纳米颗粒。将聚合物组合物的水溶液添加到革兰氏阳性菌和革兰氏阴性菌的悬浮液中，主要结果如下：(a) 添加二元聚络合物后，20小时后30%或更多的细胞存活。(b) 三元络合物杀死了 细菌，但对 细菌无效。(c) 当添加含有银纳米颗粒的三元络合物时，两种类型的存活细胞百分比均不超过0.03%。所获得的结果对抗菌制剂的开发具有重要价值。
