Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants (Ministry of Education), Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Collaborative Innovation Center, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2024 Sep 25;25(19):10312. doi: 10.3390/ijms251910312.
Plants generate a range of physiological and molecular responses to sustain their growth and development when suffering heat stress. Avocado is a type of tropical fruit tree with high economic value. Most avocado cultivars delete, wither, or even die when exposed to heat stress for a long time, which seriously restricts the introduction and cultivation of avocados. In this study, samples of a heat-intolerant variety ('') were treated under heat stress, and the transcriptomics and metabolomics were analyzed, with the expectation of providing information on the variety improvement and domestication of avocados. The differentially expressed genes identified using transcriptome analysis mainly involved metabolic pathways such as plant hormone signal transduction, plant-pathogen interaction, and protein processing in the endoplasmic reticulum. Combined transcriptome and metabolome analysis indicated that the down-regulation of and in heat shock-like proteins may result in the reduced Trehalose and Sinapoyl aldehyde content. Metabolomics analysis results indicated that the decrease in Trehalose and Sinapoyl aldehyde content may be an important factor for heat intolerance. These results provide important clues for understanding the physiological mechanisms of adaptation to heat stress in avocados.
当植物受到热胁迫时，会产生一系列生理和分子反应来维持其生长和发育。鳄梨是一种具有高经济价值的热带果树。大多数鳄梨品种在长时间暴露于热胁迫下会枯萎、凋零甚至死亡，这严重限制了鳄梨的引种和栽培。在这项研究中，对一种耐热品种（“”）进行了热胁迫处理，并对其进行了转录组学和代谢组学分析，以期为鳄梨的品种改良和驯化提供信息。转录组分析鉴定的差异表达基因主要涉及植物激素信号转导、植物-病原体相互作用和内质网中蛋白质加工等代谢途径。转录组和代谢组联合分析表明，热休克样蛋白和的下调可能导致海藻糖和芥子醛含量降低。代谢组学分析结果表明，海藻糖和芥子醛含量的降低可能是耐热性差的一个重要因素。这些结果为理解鳄梨适应热胁迫的生理机制提供了重要线索。
