College of Agriculture, Guangxi University, Nanning 530004, China.
Guangxi Key Laboratory of Agro-Environment and Agro-Products Safety, Guangxi University, Nanning 530004, China.
Int J Mol Sci. 2024 Sep 27;25(19):10416. doi: 10.3390/ijms251910416.
Aluminum (Al) toxicity in acidic soils reduces root growth and can lead to a considerable reduction in peanut plants ( L.). The caseinolytic protease (Clp) system plays the key role in abiotic stress response. However, it is still unknown whether it is involved in peanut response to Al stress. The results from this study showed that Adenosine 5'-triphosphate (ATP)-dependent caseinolytic protease proteolytic subunit 6 (AhClpP6) in peanut plants was involved in the Al stress response through its effects on leaf photosynthesis. The expression levels in the leaf and stem significantly increased with the Al treatment times. Knockdown peanut lines accumulated significantly more Al when exposed to Al stress, which reduced leaf photosynthesis. Furthermore, in response to Al treatment, knockdown of resulted in a flattened shape of chloroplasts, disordered and flattened thylakoid, and accumulating more starch grains than those of the wild-type (WT) peanut lines. Taken together, our results suggest that regulates Al tolerance by maintaining chloroplast integrity and enhancing photosynthesis.
在酸性土壤中，铝（Al）毒性会抑制根系生长，导致花生产量大幅下降。酪蛋白水解酶（Clp）系统在非生物胁迫响应中起着关键作用。然而，目前尚不清楚它是否参与了花生对铝胁迫的响应。本研究结果表明，花生植物中的腺苷 5′-三磷酸（ATP）依赖性酪蛋白水解酶蛋白酶亚基 6（AhClpP6）通过影响叶片光合作用参与铝胁迫响应。随着铝处理时间的增加，叶片和茎中的表达水平显著增加。在铝胁迫下，敲低的花生品系积累的铝明显更多，从而降低了叶片光合作用。此外，在响应铝处理时，与野生型（WT）花生品系相比，敲低的品系导致叶绿体形状变平、类囊体排列紊乱且变平，并积累了更多的淀粉粒。综上所述，我们的研究结果表明，通过维持叶绿体完整性和增强光合作用， 调节铝耐受性。
