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NanoLuc 荧光素酶作为 GFP 生色团基荧光蛋白的荧光激活蛋白。

NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens.

机构信息

Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.

Laboratory of Medicinal Substances Chemistry, Institute of Translational Medicine, Pirogov Russian National Research Medical University, Ostrovitianov 1, 117997 Moscow, Russia.

出版信息

Int J Mol Sci. 2023 Apr 27;24(9):7958. doi: 10.3390/ijms24097958.

DOI:10.3390/ijms24097958
PMID:37175667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10178166/
Abstract

In this work, we showed that the well-known NanoLuc luciferase can act as a fluorogen activating protein for various arylidene-imidazolones structurally similar to the Kaede protein chromophore. We showed that such compounds can be used as fluorescent sensors for this protein and can also be used in pairs with it in fluorescent microscopy as a genetically encoded tag.

摘要

在这项工作中，我们表明，众所周知的 NanoLuc 荧光素酶可以作为一种荧光蛋白激活蛋白，用于各种与 Kaede 蛋白发色团结构相似的芳基亚胺唑类化合物。我们表明，此类化合物可用作该蛋白质的荧光传感器，并且也可以与该蛋白质配对使用，作为荧光显微镜中的一种遗传编码标签。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/853205930f8a/ijms-24-07958-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/b967c770934f/ijms-24-07958-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/d52197a29cb1/ijms-24-07958-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/7eae674da088/ijms-24-07958-sch003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/d463653af195/ijms-24-07958-sch004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/613c06c97e9c/ijms-24-07958-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/5202f18fbccd/ijms-24-07958-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/853205930f8a/ijms-24-07958-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/b967c770934f/ijms-24-07958-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/d52197a29cb1/ijms-24-07958-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/7eae674da088/ijms-24-07958-sch003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/d463653af195/ijms-24-07958-sch004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/613c06c97e9c/ijms-24-07958-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/5202f18fbccd/ijms-24-07958-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a51/10178166/853205930f8a/ijms-24-07958-g003.jpg

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本文引用的文献

1
Color Tuning of Fluorogens for FAST Fluorogen-Activating Protein.荧光团的颜色调谐用于 FAST 荧光素激活蛋白。
Chemistry. 2021 Feb 24;27(12):3986-3990. doi: 10.1002/chem.202004760. Epub 2021 Feb 2.
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Red-Shifted Substrates for FAST Fluorogen-Activating Protein Based on the GFP-Like Chromophores.基于 GFP 类似发色团的 FAST 荧光素酶激活蛋白的红移底物。
Chemistry. 2019 Jul 22;25(41):9592-9596. doi: 10.1002/chem.201901151. Epub 2019 Jul 1.
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De novo design of a fluorescence-activating β-barrel.从头设计一个荧光激活的β桶。
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Protein labeling for live cell fluorescence microscopy with a highly photostable renewable signal.用于活细胞荧光显微镜检查的具有高光稳定性可再生信号的蛋白质标记。
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DNA mimics of red fluorescent proteins (RFP) based on G-quadruplex-confined synthetic RFP chromophores.基于G-四链体限制的合成红色荧光蛋白发色团的红色荧光蛋白的DNA模拟物。
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Solvatochromic and Fluorogenic Dyes as Environment-Sensitive Probes: Design and Biological Applications.溶剂化变色和荧光染料作为环境敏感探针：设计与生物学应用。
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NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence.纳米荧光素酶：一种小型荧光素酶正在照亮生物发光领域。
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