Institute of Physics, University of Silesia in Katowice, 75 Pułku Piechoty 1A, 41-500 Chorzow, Poland.
SPIN-Lab Centre for Microscopic Research of Matter, University of Silesia in Katowice, 75 Pułku Piechoty 1A, 41-500 Chorzow, Poland.
Int J Mol Sci. 2024 Sep 28;25(19):10468. doi: 10.3390/ijms251910468.
Iron plays a crucial role in various metabolic processes. However, the impact of 5-aminolevulinic acid (ALA) in combination with iron chelators on iron metabolism and the efficacy of ALA-photodynamic therapy (PDT) remain inadequately understood. This study aimed to examine the effect of thiosemicarbazone derivatives during ALA treatment on specific genes related to iron metabolism, with a particular emphasis on mitochondrial iron metabolism genes. In our study, we observed differences depending on the cell line studied. For the HCT116 and MCF-7 cell lines, in most cases, the decrease in the expression of selected targets correlated with the increase in protoporphyrin IX (PPIX) concentration and the observed photodynamic effect, aligning with existing literature data. The Hs683 cell line showed a different gene expression pattern, previously not described in the literature. In this study, we collected an extensive analysis of the gene variation occurring after the application of novel thiosemicarbazone derivatives and presented versatile and effective compounds with great potential for use in ALA-PDT.
铁在各种代谢过程中起着至关重要的作用。然而，5-氨基酮戊酸（ALA）与铁螯合剂联合应用对铁代谢的影响以及 ALA 光动力疗法（PDT）的疗效仍了解不足。本研究旨在研究 ALA 治疗期间使用噻唑烷酮衍生物对与铁代谢相关的特定基因的影响，特别关注线粒体铁代谢基因。在我们的研究中，我们观察到不同细胞系的结果存在差异。对于 HCT116 和 MCF-7 细胞系，在大多数情况下，所选靶标表达的降低与原卟啉 IX（PPIX）浓度的增加和观察到的光动力效应相关，与现有文献数据一致。Hs683 细胞系表现出不同的基因表达模式，这在文献中尚未描述。在这项研究中，我们对新型噻唑烷酮衍生物应用后发生的基因变异进行了广泛的分析，并提出了具有广泛用途的有效化合物，这些化合物在 ALA-PDT 中有很大的应用潜力。
