Department of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, Seoul 05029, Republic of Korea.
R&D Team, StemExOne Co., Ltd., 307, KU Technology Innovation Building, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
Int J Mol Sci. 2024 Oct 4;25(19):10709. doi: 10.3390/ijms251910709.
The skin is an essential organ that protects the body from external aggressions; therefore, damage from various wounds can significantly impair its function, and effective methods for regenerating and restoring its barrier function are crucial. This study aimed to mass-produce wound-healing exosomes using a fragment of the fibroblast growth factor 2 (FGF2)-derived peptide (FP2) to enhance cell proliferation and exosome production. Our experiments demonstrated increased cell proliferation when Wharton's jelly mesenchymal stem cells (WJ MSCs) were coated with FP2. Exosomes from FP2-coated WJ MSCs were analyzed using nanoparticle-tracking analysis, transmission electron microscopy, and Western blotting. Subsequently, fibroblasts were treated with these exosomes, and their viability and migration effects were compared. Anti-inflammatory effects were also evaluated by inducing pro-inflammatory factors in RAW264.7 cells. The treatment of fibroblasts with FP2-coated WJ MSC-derived exosomes (FP2-exo) increased the expression of FGF2, confirming their wound-healing effect in vivo. Overall, the results of this study highlight the significant impact of FP2 on the proliferation of WJ MSCs and the anti-inflammatory and wound-healing effects of exosomes, suggesting potential applications beyond wound healing.
皮肤是人体重要的器官，它可以保护身体免受外界侵害。因此，各种创伤造成的损伤会显著损害其功能，而有效再生和恢复其屏障功能的方法至关重要。本研究旨在利用成纤维细胞生长因子 2（FGF2）衍生肽（FP2）的片段大量生产伤口愈合外泌体，以增强细胞增殖和外泌体的产生。我们的实验表明，用 FP2 包被的牙髓间充质干细胞（WJ MSC）可增加细胞增殖。使用纳米颗粒跟踪分析、透射电子显微镜和 Western blot 分析 FP2 包被的 WJ MSC 来源的外泌体。然后用这些外泌体处理成纤维细胞，并比较其活力和迁移效应。通过在 RAW264.7 细胞中诱导促炎因子来评估抗炎作用。用 FP2 包被的 WJ MSC 衍生的外泌体（FP2-exo）处理成纤维细胞，增加了 FGF2 的表达，证实了其在体内的伤口愈合作用。总的来说，这项研究的结果突出了 FP2 对 WJ MSC 增殖以及外泌体抗炎和伤口愈合作用的显著影响，表明其在伤口愈合之外的潜在应用。
