Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav St., 1000 Sofia, Bulgaria.
Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav St., 1000 Sofia, Bulgaria.
Int J Mol Sci. 2024 Oct 9;25(19):10851. doi: 10.3390/ijms251910851.
Metabolic disorders (MDs) include disease states such as diabetes mellitus, obesity, dyslipidemia, hyperuricemia, etc., affecting about 30% of the planet's population. The purpose of the present study was to investigate the protective potential of leaf extract (ECA) against chemically induced type 2 diabetes in Wistar rats. Additionally, some biochemical parameters in the blood serum and liver, as well as histopathological investigation, were also performed. Quantitative analysis of the major compounds in the used extract was performed using ultrahigh-performance liquid chromatography-diode array detection (UHPLC-DAD) analyses using the external standard method. extract revealed a beneficial effect on MDs, lowering blood sugar levels and MDA quantity in the liver, increasing the reduced glutathione level, and increasing antioxidant enzyme activity. Cichoric acid (CA) (91.93 mg/g dry extract (de) ± 4.64 mg/g de) was found to be the dominant compound in the extract, followed by caftaric (11.36 ± 2.10 mg/g de), and chlorogenic acid (CGA) (9.25 ± 0.05 mg/g de). In conclusion, leaf extract (ECA) is rich in caffeoyltartaric and caffeoylquinic acids and provides beneficial effects on the diabetic animal model.
代谢紊乱（MDs）包括糖尿病、肥胖症、血脂异常、高尿酸血症等疾病状态，影响了全球约 30%的人口。本研究旨在探讨叶提取物（ECA）对 Wistar 大鼠化学诱导型 2 型糖尿病的保护潜力。此外，还对血清和肝脏中的一些生化参数以及组织病理学进行了研究。采用超高效液相色谱-二极管阵列检测（UHPLC-DAD）分析，采用外标法对所用提取物中的主要化合物进行定量分析。结果表明，叶提取物对 MDs 具有有益的作用，降低了血糖水平和肝脏中的 MDA 含量，增加了还原型谷胱甘肽水平，并提高了抗氧化酶的活性。在提取物中发现咖啡酰酒石酸（CA）（91.93 mg/g 干提取物（de）±4.64 mg/g de）是主要化合物，其次是咖啡酸（11.36±2.10 mg/g de）和绿原酸（CGA）（9.25±0.05 mg/g de）。综上所述，叶提取物（ECA）富含咖啡酰酒石酸和咖啡酰奎宁酸，对糖尿病动物模型具有有益的作用。
