洋蓟叶提取物对四氧嘧啶糖尿病大鼠代谢紊乱和氧化应激的保护作用。
Protective effects of Cynara scolymus leaves extract on metabolic disorders and oxidative stress in alloxan-diabetic rats.
作者信息
Ben Salem Maryem, Ben Abdallah Kolsi Rihab, Dhouibi Raouia, Ksouda Kamilia, Charfi Slim, Yaich Mahdi, Hammami Serria, Sahnoun Zouheir, Zeghal Khaled Mounir, Jamoussi Kamel, Affes Hanen
机构信息
Laboratory of pharmacology, Faculty of Medicine, University of Sfax, Avenue Majida Boulila, 3029, Sfax, Tunisia.
Laboratory of Plant Biotechnology, Faculty of Sciences, University of Sfax, Sfax, Tunisia.
出版信息
BMC Complement Altern Med. 2017 Jun 19;17(1):328. doi: 10.1186/s12906-017-1835-8.
BACKGROUND
Diabetes mellitus (DM) is associated with hyperglycemia, inflammatory disorders and abnormal lipid profiles, currently the extracts from leaves of cynara scolymus has been discovered to treat metabolic disorders and has been stated by multitudinous scientists according to a good source of polyphenols compounds. The present study aimed to evaluate the protective effect of the ethanol leaves extract of C. scolymus in alloxan induced stress oxidant, hepatic-kidney dysfunction and histological changes in liver, kidney and pancreas of different experimental groups of rats.
METHODS
We determinate the antioxidant activity by ABTS and antioxidant total capacity (TAC) of all extracts of C. scolymus leaves, the inhibition of α-amylase activity in vitro was also investigated. Forty male Wistar rats were induced to diabetes with a single dose intraperitoneal injection (i.p.) of alloxan (150 mg/kg body weight (b.w.)). Diabetic rats were orally and daily administrated of ethanol extract from C. scolymus at two doses (200-400 mg/kg, b.w) or (12 mg/kg, b.w) with anti-diabetic reference drug, Acarbose for one month. Ethanol extract of C. scolymus effect was confirmed by biochemical analysis, antioxidant activity and histological study.
RESULTS
The results indicated that the ethanol extract from leaves of C. scolymus showed the highest antioxidant activity by ABTS (499.43g± 39.72 Trolox/g dry extract) and (128.75 ± 8.45 mg VC /g dry extract) for TAC and endowed the powerful inhibition in vitro of α-amylase activity with IC50=72,22 ug/uL. In vivo, the results showed that ethanol extract from the leaves of C. scolymus (200-400 mg/kg) decreased significantly (p < 0.001) the α-amylase levels in serum of diabetic rats, respectively associated with significant reduction (p < 0.001) in blood glucose rate of 42,84% and 37,91% compared to diabetic groups after 28 days of treatment, a significant lowered of plasma total cholesterol (T-Ch) by 18,11% and triglyceride (TG) by 60,47%, significantly and low-density lipoproteins (LDL-C) by 37,77%, compared to diabetic rats, moreover, the administration of ethanol extract appears to exert anti-oxidative activity demonstrated by the increase of CAT, SOD and GSH activities in liver, kidney and pancreas of diabetic rats. This positive effect of the ethanol extract from C. scolymus was confirmed by histological study.
CONCLUSION
These observed strongly suggest that ethanol extract from the leaves of C. scolymus has anti-hyperglycemic properties, at least partly mediated by antioxidant and hypolipidemic effects.
背景
糖尿病（DM）与高血糖、炎症紊乱及血脂异常有关，目前已发现洋蓟叶提取物可治疗代谢紊乱，众多科学家称其是多酚类化合物的优质来源。本研究旨在评估洋蓟叶乙醇提取物对四氧嘧啶诱导的应激氧化、肝肾损伤以及不同实验组大鼠肝脏、肾脏和胰腺组织学变化的保护作用。
方法
我们通过ABTS法测定了洋蓟叶所有提取物的抗氧化活性及总抗氧化能力（TAC），还研究了其对体外α -淀粉酶活性的抑制作用。40只雄性Wistar大鼠通过腹腔注射（i.p.）单剂量四氧嘧啶（150 mg/kg体重（b.w.））诱导糖尿病。糖尿病大鼠每天口服两种剂量（200 - 400 mg/kg，b.w）或（12 mg/kg，b.w）的洋蓟乙醇提取物以及抗糖尿病参考药物阿卡波糖，持续一个月。通过生化分析、抗氧化活性和组织学研究来证实洋蓟乙醇提取物的作用。
结果
结果表明，洋蓟叶乙醇提取物通过ABTS法显示出最高的抗氧化活性（499.43g±39.72 Trolox/g干提取物），TAC为（128.75±8.45 mg VC /g干提取物），并且在体外对α -淀粉酶活性具有强大的抑制作用，IC50 = 72.22 ug/uL。在体内，结果显示洋蓟叶乙醇提取物（200 - 400 mg/kg）分别显著降低（p < 0.001）糖尿病大鼠血清中的α -淀粉酶水平，与糖尿病组相比，治疗28天后血糖率分别显著降低（p < 0.001）42.84%和37.91%，血浆总胆固醇（T - Ch）显著降低18.11%，甘油三酯（TG）降低60.47%，低密度脂蛋白（LDL - C）降低37.77%。此外，给予乙醇提取物似乎具有抗氧化活性，表现为糖尿病大鼠肝脏、肾脏和胰腺中CAT、SOD和GSH活性增加。洋蓟乙醇提取物的这种积极作用通过组织学研究得到证实。
结论
这些观察结果强烈表明，洋蓟叶乙醇提取物具有降血糖特性，至少部分是由抗氧化和降血脂作用介导的。
Zotero 插件