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大蒜(L.)中家族的鉴定及盐胁迫下的验证

Identification of the Family in Garlic ( L.) and Verification of the under Salt Stress.

作者信息

Peng Xianghan, Ruan Jiaojiao, Jiang Fangling, Zhou Rong, Wu Zhen

机构信息

College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.

Department of Food Science, Aarhus University, Agro Food Park 48, 8200 Aarhus, Denmark.

出版信息

Plants (Basel). 2024 Sep 30;13(19):2749. doi: 10.3390/plants13192749.

Abstract

Brassinazole-Resistant (BZR) is an important transcription factor (TF) in the brassinosteroid (BR) signaling pathway, which plays a crucial role in plant growth, development and stress resistance. In this study, we performed a genome-wide analysis of in garlic ( L.) and identified a total of 11 members of the gene family. By comparing the expression patterns of genes under salt stress, the candidate gene with salt tolerance function was identified. Subcellular localization results showed that was localized in the nucleus. The salt tolerance of overexpression lines improved, and the germination rate and root length of overexpression lines increased as compared with wild type. The content of reactive oxygen species (ROS) decreased, and the activity of antioxidant enzymes increased in -OE, suggesting that has the function of improving plant salt tolerance. Our results enriched the knowledge of plant family and laid a foundation for the molecular mechanism of salt tolerance of garlic, which will provide a theoretical basis for the subsequent creation of salt-tolerant germplasm resources.

摘要

油菜素唑抗性(BZR)是油菜素甾醇(BR)信号通路中的一种重要转录因子(TF),在植物生长、发育和抗逆性中起关键作用。在本研究中,我们对大蒜( )进行了全基因组分析,共鉴定出 基因家族的11个成员。通过比较盐胁迫下 基因的表达模式,鉴定出具有耐盐功能的候选基因 。亚细胞定位结果表明 定位于细胞核。过表达株系的耐盐性提高,与野生型相比,过表达株系的发芽率和根长增加。 -OE中活性氧(ROS)含量降低,抗氧化酶活性增加,表明 具有提高植物耐盐性的功能。我们的研究结果丰富了植物 家族的知识,为大蒜耐盐分子机制奠定了基础,将为后续耐盐种质资源的创制提供理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aff/11478727/70a01bb7dbd3/plants-13-02749-g001.jpg

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