College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.
Liaoning Province Modern Agricultural Production Base Construction Engineering Center, Liaoyang, China.
Anim Biotechnol. 2023 Jun;34(3):698-708. doi: 10.1080/10495398.2021.1996386. Epub 2021 Nov 8.
Cashmere fineness is getting thicker, which is one of the key problems in cashmere breeding, however, there have been no systematic studies on the molecular regulation of cashmere fineness. The aim of this study was to investigate the relationship between and gene polymorphism and production performance in Liaoning cashmere goats (LCG). The potential single nucleotide polymorphisms (SNPs) of LCG were detected by sequence alignment and PCR-Seq polymorphism of and genes and analyzed the effect of SNPs on production performance by SPSS software. Two SNPs sites (A559T and A6839G) of two genes were detected. The AA genotype of A559T locus was the dominant genotype. AG and GG at A6839G locus were the dominant genotypes. AAAA was the dominant haplotype combination. The results showed that and genes were associated with cashmere fineness of LCG, and A559T (AA) and A6839G (GG) genotypes were the preferred marker genotypes for cashmere fineness, which provided more theoretical basis for further research on cashmere fineness.
羊绒细度变粗，是羊绒育种的关键问题之一，但目前尚无关于羊绒细度分子调控的系统研究。本研究旨在探讨辽宁绒山羊（LCG） 基因和 基因多态性与生产性能的关系。通过序列比对和 PCR-Seq 对 LCG 的潜在单核苷酸多态性（SNP）进行检测，并通过 SPSS 软件分析 SNP 对生产性能的影响。在两个基因中检测到两个 SNP 位点（A559T 和 A6839G）。A559T 位点的 AA 基因型是优势基因型。A6839G 位点的 AG 和 GG 是优势基因型。AAAA 是优势单倍型组合。结果表明， 和 基因与 LCG 的羊绒细度有关，A559T（AA）和 A6839G（GG）基因型是羊绒细度的首选标记基因型，为进一步研究羊绒细度提供了更多的理论依据。
