Milfort Marie C, Ghareeb Ahmed F A, Ariyo Oluwatomide W, Kwakye Josephine, Hartono Evan, Sovi Selorm, Aryal Bikash, Fuller Alberta L, El Sabry Mohamed I, Stino Farid, Rekaya Romdhane, Aggrey Samuel E
Water Intake Genomics Laboratory, Department of Poultry Science, University of Georgia, Athens, GA 30602, USA.
Department of Animal Production, Cairo University, Giza 12613, Egypt.
Animals (Basel). 2024 Oct 9;14(19):2912. doi: 10.3390/ani14192912.
Molecular differences exist between birds with high residual water intake (HRWI) compared to those with low residual water intake (LRWI). Residual water intake (RWI) is defined as the difference between the water intake of a bird and the expected water intake corrected for metabolic body weight, feed intake, and body weight gain. Tissue metabolomic analysis revealed significantly increased kidney glucose, fructose, and arabitol in the LRWI group compared to the HRWI group. mRNA expression analysis of apical sodium glucose cotransporters SGLT1, SGLT4, SGLT5, and SGLT6 showed decreased expression of SGLTs 1, 5, and 6 in LRWI birds ( < 0.05), whereas SGLT4 expression was increased compared with HRWI birds ( < 0.01). An analysis of basal glucose transporters GLUT1, GLUT2, GLUT5, and GLUT9 showed significantly increased GLUT2 expression in LRWI birds compared with HRWI birds ( < 0.01). We postulate that SGLT4 is the main apical transporter in chicken kidneys and that its increased expression reduces these birds' need for water, resulting in less drinking. This is balanced by the increased expression of the basal transporter GLUT2, indicating better glucose retention, which may partly explain the physiological mechanism behind why these birds drink less water. Innately driven broiler water intake could therefore be influenced by the expression of kidney solute transporters.
与低残余水摄入量（LRWI）的鸟类相比，高残余水摄入量（HRWI）的鸟类之间存在分子差异。残余水摄入量（RWI）定义为鸟类的水摄入量与根据代谢体重、采食量和体重增加校正后的预期水摄入量之间的差值。组织代谢组学分析显示，与HRWI组相比，LRWI组的肾脏葡萄糖、果糖和阿拉伯糖醇显著增加。顶端钠葡萄糖共转运蛋白SGLT1、SGLT4、SGLT5和SGLT6的mRNA表达分析表明，LRWI鸟类中SGLT1、5和6的表达降低（<0.05），而与HRWI鸟类相比，SGLT4的表达增加（<0.01）。对基础葡萄糖转运蛋白GLUT1、GLUT2、GLUT5和GLUT9的分析表明，与HRWI鸟类相比，LRWI鸟类中GLUT2的表达显著增加（<0.01）。我们推测SGLT4是鸡肾脏中的主要顶端转运蛋白，其表达增加降低了这些鸟类对水的需求，导致饮水量减少。这通过基础转运蛋白GLUT2表达的增加来平衡，表明更好的葡萄糖保留，这可能部分解释了这些鸟类饮水量较少背后的生理机制。因此，肉鸡天生的饮水量可能受肾脏溶质转运蛋白表达的影响。
