Zheng Qinri, Chia Suet Lin, Saad Norazalina, Song Adelene Ai-Lian, Loh Teck Chwen, Foo Hooi Ling
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
UPM-MAKNA Cancer Research Laboratory (CANRES), Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Foods. 2024 Sep 30;13(19):3123. doi: 10.3390/foods13193123.
Postbiotic metabolites produced by strains isolated from Malaysian food have been extensively reported for their positive effects on health. Understanding the effects of different combinations of carbon and nitrogen sources on the growth and corresponding characteristics of postbiotic metabolites produced by different strains of is important for various applications. Hence, the effects of different combinations of carbon (glucose, lactose, sucrose and dextrose) and nitrogen (X-SEED Kat, X-SEED Peptone, X-SEED Nucleo Advanced, Nucel875 MG, FM888 and FM902) sources on the growth of six strains of (RG11, RG14, RI11, RS5, TL1 and UL4) and the functional characteristics (bacteriocin inhibitory activity, antioxidant activity and lactic acid concentration) of their respective postbiotic metabolites were investigated in this study. UL4 produced the highest viable cell population with sucrose and Nucel875 nitrogen source. The UL4 strain also produced the strongest bacteriocin inhibitory activity with dextrose and FM888 nitrogen source. In comparison, the RI11 strain produced the highest lactic acid concentration with dextrose and Nucel875 nitrogen source and the highest reducing power of RS5 and TL1 postbiotic metabolites was achieved with MRS medium. In the combination of sucrose and X-Seed KAT nitrogen source, RG14 produce the highest hydroxyl radical scavenging activity. The effects of different combinations of carbon and nitrogen sources on the viable cell population of strains and the respective functional characteristics of postbiotic metabolites were strain dependent. The current study also revealed that fermentation media were an important factor that greatly impacted the functionalities of postbiotic metabolites due to the presence of various bioactive compounds that contributed to high antioxidant and antimicrobial properties. The results of this study will facilitate the subsequent medium design and optimisation for the development and production of specific postbiotic metabolites produced by the respective . strain for their applications in various industries.
从马来西亚食品中分离出的菌株产生的后生元代谢产物对健康的积极影响已被广泛报道。了解不同碳源和氮源组合对不同菌株产生的后生元代谢产物的生长及相应特性的影响，对于各种应用来说至关重要。因此，本研究考察了碳源（葡萄糖、乳糖、蔗糖和右旋糖）和氮源（X-SEED Kat、X-SEED蛋白胨、X-SEED Nucleo Advanced、Nucel875 MG、FM888和FM902）的不同组合对六种 菌株（RG11、RG14、RI11、RS5、TL1和UL4）生长的影响以及它们各自后生元代谢产物的功能特性（细菌素抑制活性、抗氧化活性和乳酸浓度）。UL4在蔗糖和Nucel875氮源条件下产生的活菌数最高。UL4菌株在右旋糖和FM888氮源条件下还产生了最强的细菌素抑制活性。相比之下，RI11菌株在右旋糖和Nucel875氮源条件下产生的乳酸浓度最高，而RS5和TL1后生元代谢产物的最高还原力是在MRS培养基中实现的。在蔗糖和X-Seed KAT氮源组合中，RG14产生的羟自由基清除活性最高。碳源和氮源的不同组合对 菌株活菌数以及后生元代谢产物各自功能特性的影响因菌株而异。当前研究还表明，发酵培养基是一个重要因素，由于存在各种有助于提高抗氧化和抗菌性能的生物活性化合物，它对后生元代谢产物的功能有很大影响。本研究结果将有助于后续培养基的设计和优化，以开发和生产由各自 菌株产生的特定后生元代谢产物，用于各种行业应用。
