Zhang Yijun, Hua Tianfeng, Huang Xiaoyi, Gu Rongrong, Chu Ruixi, Hu Yan, Ye Sheng, Yang Min
The Second Department of Critical Care Medicine, The Second Affiliated Hospital of Anhui Medical University Hefei Anhui 230001 China
Laboratory of Cardiopulmonary Resuscitation and Critical Care, The Second Affiliated Hospital of Anhui Medical University Hefei Anhui 230001 China.
RSC Adv. 2024 Oct 15;14(44):32533-32541. doi: 10.1039/d4ra04157g. eCollection 2024 Oct 9.
Ischemia-reperfusion injury resulting from severe hemorrhagic shock continues to cause substantial damage to human health and impose a significant economic burden. In this study, we designed an Au-loaded yolk-shell MoS nanoreactor (Au@MoS) that regulates cellular homeostasis. experiments validated the efficacy of the nanomaterial in reducing intracellular reactive oxygen species (ROS) production during hypoxia and reoxygenation, and had great cell biocompatibility, Au@MoS. The antioxidant properties of the nanoreactors contributed to the elimination of ROS (over twofold scavenging ratio for ROS). results demonstrate that Au@MoS (54.88% of reduction) alleviates hyperlactatemia and reduces ischemia-reperfusion injury in rats subjected to severe hemorrhagic shock, compared to MoS (26.32% of reduction) alone. In addition, no discernible toxic side effects were observed in the rats throughout the experiment, underscoring the considerable promise of the nanoreactor for clinical trials. The mechanism involves catalyzing the degradation of endogenous lactic acid on the Au@MoS nanoreactor under 808 nm light, thereby alleviating ischemia-reperfusion injury. This work proposes a new selective strategy for the treatment of synergistic hemorrhagic shock.
严重失血性休克导致的缺血再灌注损伤持续对人类健康造成重大损害，并带来巨大的经济负担。在本研究中，我们设计了一种负载金的蛋黄壳型二硫化钼纳米反应器（Au@MoS），其可调节细胞内稳态。实验验证了该纳米材料在缺氧和复氧过程中减少细胞内活性氧（ROS）产生的功效，并且Au@MoS具有良好的细胞生物相容性。纳米反应器的抗氧化特性有助于清除ROS（对ROS的清除率超过两倍）。结果表明，与单独的二硫化钼（减少26.32%）相比，Au@MoS（减少54.88%）可减轻严重失血性休克大鼠的高乳酸血症并减少缺血再灌注损伤。此外，在整个实验过程中未在大鼠中观察到明显的毒副作用，这突出了该纳米反应器在临床试验中的巨大前景。其机制包括在808nm光下催化Au@MoS纳米反应器上内源性乳酸的降解，从而减轻缺血再灌注损伤。这项工作为协同性失血性休克的治疗提出了一种新的选择性策略。
