Bieler E U, Pitout M J, Stroud S W, van Rooyen R J
Horm Res. 1977;8(1):29-36. doi: 10.1159/000178777.
Monomeric human growth hormore (hGH) and big hGH obtained by a new purification procedure from human pituitary glands were examined for a possible conversion into different molecular weight forms under in vitro and in vivo conditions. Sephadex G-150 gel filtration was used for separation. Big or monomeric hGH was incubated in hormone-free serum or injected into normal individuals in order to induce a peak of immunoreactive hGH. Specimens from early and late stages of the surge were subjected to gel filtration. It could be shown that not only the big hGH is converted to the smaller form but that monomeric hGH is also recovered in two higher molecular weight regions by gel filtration in vitro and in vivo experiments. A significantly higher conversion of monomeric to big hGH was found in vitro. This suggests an inhibition of big hGH formation from monomeric hGH in normal individuals. There was no apparent time relation of big hGH formation from monomeric hGH in vivo. Regarding the conversion of big hGH, more of this material remained in its original form in vitro. The immunoreactivity recovered in the region of the very large form was found to be higher in vivo. The formation of the monomer from big hGH was similar under in vitro and in vivo conditions.
通过一种新的纯化方法从人垂体中获得的单体人生长激素（hGH）和大hGH，在体外和体内条件下检测了其转化为不同分子量形式的可能性。使用Sephadex G - 150凝胶过滤进行分离。将大hGH或单体hGH在无激素血清中孵育或注射到正常个体中以诱导免疫反应性hGH峰值。对激增早期和晚期的样本进行凝胶过滤。结果表明，不仅大hGH会转化为较小形式，而且在体外和体内实验中，单体hGH通过凝胶过滤也会在两个较高分子量区域中被回收。在体外发现单体hGH向大hGH的转化率明显更高。这表明在正常个体中，单体hGH形成大hGH的过程受到抑制。在体内，单体hGH形成大hGH没有明显的时间关系。关于大hGH的转化，这种物质在体外更多地保持其原始形式。在体内，在非常大形式区域回收的免疫反应性更高。在体外和体内条件下，大hGH形成单体的情况相似。
