石榴（重瓣石榴品种）花（戈尔纳尔）对引起食源性疾病的细菌菌株的总酚和黄酮含量及抗菌活性。
Total phenolic and flavonoid content and antibacterial activity of Punica granatum L. var. pleniflora flowers (Golnar) against bacterial strains causing foodborne diseases.
作者信息
Mahboubi Arash, Asgarpanah Jinous, Sadaghiyani Parisa Nosrati, Faizi Mehrdad
机构信息
Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, 2660, Vali-Asr Ave, Tehran, 141556153, Iran.
Young Researchers and Elite Club, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
出版信息
BMC Complement Altern Med. 2015 Oct 15;15:366. doi: 10.1186/s12906-015-0887-x.
BACKGROUND
Flowers of Punica granatum L. (Punicaceae) var. pleniflora, known as "Golnar" in Iranian traditional medicine have been used for the prevention and treatment of foodborne diseases. In this study, antibacterial activities of ethanol extract of Golnar and its fractions were scientifically evaluated against bacteria causing foodborne diseases including Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli, Shigella dysantriae, and Salmonella typhi. The total phenolic and flavonoid contents of the extract and its fractions were also determined.
METHODS
The antibacterial effect of the ethanol extract and its fractions were primarily evaluated by agar well diffusion and their MIC and MBC were determined by broth macro dilution method. The total phenolic and flavonoid contents of the extract and its fractions were measured based on gallic acid and rutin equivalents (GAE and RE), respectively.
RESULTS
After evaluation of total phenolic and flavonoid content the chloroform fraction showed the lowest phenolic and flavonoid contents (3.8 mg GAE/g and 1.1 mg RE/g respectively) and the methanol fraction showed the highest phenolic and flavonoid contents (18.1 mg GEA/g and 3.3 mg RE/g respectively). The total phenolic and flavonoid content was positively associated with the antibacterial activities of the fractions with chloroform extract exhibiting lowest antibacterial activity against E. coli (MIC 25 mg/ml) and the methanol fraction exhibiting the highest antibacterial effect against S. aureus (MIC 0.19 mg/ml).
CONCLUSION
Golnar extract showed antibacterial activity against both Gram positive and Gram negative bacteria causing food poisoning. Therefore, the extract can be used for prevention or treatment of foodborne diseases or as preservative in the food industry. The methanol fraction with the highest phenolic and flavonoid content showed the highest antibacterial effect. This indicates that the phenolic and flavonoid compounds in the extract can be responsible for the effect.
背景
石榴科石榴（Punica granatum L.）重瓣花品种，在伊朗传统医学中被称为“戈尔纳尔”，已被用于预防和治疗食源性疾病。在本研究中，对戈尔纳尔乙醇提取物及其馏分对引起食源性疾病的细菌，包括金黄色葡萄球菌、蜡样芽孢杆菌、单核细胞增生李斯特菌、大肠杆菌、痢疾志贺菌和伤寒沙门氏菌的抗菌活性进行了科学评估。还测定了提取物及其馏分的总酚和黄酮含量。
方法
通过琼脂孔扩散法初步评估乙醇提取物及其馏分的抗菌效果，并通过肉汤宏观稀释法测定其最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。提取物及其馏分的总酚和黄酮含量分别基于没食子酸和芦丁当量（GAE和RE）进行测定。
结果
在评估总酚和黄酮含量后，氯仿馏分的酚和黄酮含量最低（分别为3.8 mg GAE/g和1.1 mg RE/g），甲醇馏分的酚和黄酮含量最高（分别为18.1 mg GEA/g和3.3 mg RE/g）。总酚和黄酮含量与馏分的抗菌活性呈正相关，氯仿提取物对大肠杆菌的抗菌活性最低（MIC为25 mg/ml），甲醇馏分对金黄色葡萄球菌的抗菌效果最高（MIC为0.19 mg/ml）。
结论
戈尔纳尔提取物对引起食物中毒的革兰氏阳性菌和革兰氏阴性菌均表现出抗菌活性。因此，该提取物可用于预防或治疗食源性疾病，或作为食品工业中的防腐剂。酚和黄酮含量最高的甲醇馏分表现出最高的抗菌效果。这表明提取物中的酚类和黄酮类化合物可能是产生这种效果的原因。
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