Luo Xi, Yan Guoyong, Wang Qinggui, Xing Yajuan
School of Life Sciences, Qufu Normal University, Qufu, China.
Library, Qufu Normal University, Qufu, China.
Front Microbiol. 2024 Oct 2;15:1410901. doi: 10.3389/fmicb.2024.1410901. eCollection 2024.
Despite extensive studies on soil microbial community structure and functions, the significance of plant-associated microorganisms, especially endophytes, has been overlooked. To comprehensively anticipate future changes in forest ecosystem function under future climate change scenarios, it is imperative to gain a thorough understanding of the community structure, diversity, and function of both plant-associated microorganisms and soil microorganisms.
In our study, we aimed to elucidate the structure, diversity, and function of leaf endophytes, root endophytes, rhizosphere, and soil microbial communities in boreal forest. The microbial structure and composition were determined by high-throughput sequencing. FAPROTAX and FUNGuild were used to analyze the microbial functional groups.
Our findings revealed significant differences in the community structure and diversity of fungi and bacteria across leaves, roots, rhizosphere, and soil. Notably, we observed that the endophytic fungal or bacterial communities associated with plants comprised many species distinct from those found in the soil microbial communities, challenging the assumption that most of endophytic fungal or bacterial species in plants originate from the soil. Furthermore, our results indicated noteworthy differences in the composition functional groups of bacteria or fungi in leaf endophytes, root endophytes, rhizosphere, and soil, suggesting distinct roles played by microbial communities in plants and soil.
These findings underscore the importance of recognizing the diverse functions performed by microbial communities in both plant and soil environments. In conclusion, our study emphasizes the necessity of a comprehensive understanding of the structure and function microbial communities in both plants and soil for assessing the functions of boreal forest ecosystems.
尽管对土壤微生物群落结构和功能进行了广泛研究，但与植物相关的微生物，尤其是内生菌的重要性却被忽视了。为了全面预测未来气候变化情景下森林生态系统功能的变化，必须深入了解与植物相关的微生物和土壤微生物的群落结构、多样性及功能。
在我们的研究中，旨在阐明北方森林中叶片内生菌、根系内生菌、根际和土壤微生物群落的结构、多样性及功能。通过高通量测序确定微生物结构和组成。使用FAPROTAX和FUNGuild分析微生物功能群。
我们的研究结果揭示了真菌和细菌在叶片、根系、根际和土壤中的群落结构和多样性存在显著差异。值得注意的是，我们观察到与植物相关的内生真菌或细菌群落包含许多与土壤微生物群落中不同的物种，这对植物中大多数内生真菌或细菌物种源自土壤这一假设提出了挑战。此外，我们的结果表明叶片内生菌、根系内生菌、根际和土壤中细菌或真菌的组成功能群存在显著差异，表明微生物群落在植物和土壤中发挥着不同的作用。
这些发现强调了认识微生物群落在植物和土壤环境中所执行的多种功能的重要性。总之，我们的研究强调了全面了解植物和土壤中微生物群落的结构和功能对于评估北方森林生态系统功能的必要性。
