School of Radiology, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, Shandong Province, China.
Department of Cardiology, The Second Affiliated Hospital of Shandong First Medical University, Tai'an, Shandong Province, China.
Braz J Med Biol Res. 2024 Oct 14;57:e14345. doi: 10.1590/1414-431X2024e14345. eCollection 2024.
Acute myocardial infarction (AMI) continues to be a leading cause of death globally, with distinct immune cell dynamics in ST-segment elevation myocardial infarction (STEMI) and non-ST-segment elevation myocardial infarction (NSTEMI) playing a critical role in disease progression and patient outcomes. Sample data for STEMI and NSTEMI were downloaded from the Sequence Read Archive (SRA) database (https://www.ncbi.nlm.nih.gov/sra). Differences and correlations of immune infiltrating cells were assessed by CIBERSORT. Differentially expressed genes (DEGs) were identified between STEMI and NSTEMI, followed by functional analysis. Immune-related DEGs were further identified. Some immune-related DEGs were selected to perform expression verification using real-time PCR. There was a significant difference in immune cells between STEMI and NSTEMI, including activated dendritic cells, memory CD4 T cells, mast cells, and CD8 T cells. A total of 229 DEGs were identified, with functions related to inflammatory regulation and drug metabolism. A total of 21 immune-related DEGs, which may play important roles in STEMI and NSTEMI, were identified. Among the 21 immune-related DEGs, genes like CCL18, NRP2, CXCR2, CXCL9, KIR2DL4, BPIFB1, and IL33 were significantly correlated with immune cells and had a tendency for differential expression between STEMI and NSTEMI patients. Our study reveals differences in the distribution of immune cell subsets between STEMI and NSTEMI, highlighting key immune-related genes and their association with immune cells, which may provide new insights into the treatment of AMI.
急性心肌梗死（AMI）仍然是全球主要的死亡原因，ST 段抬高型心肌梗死（STEMI）和非 ST 段抬高型心肌梗死（NSTEMI）中独特的免疫细胞动力学在疾病进展和患者预后中起着关键作用。STEMI 和 NSTEMI 的样本数据从序列读取档案（SRA）数据库（https://www.ncbi.nlm.nih.gov/sra）下载。通过 CIBERSORT 评估免疫浸润细胞的差异和相关性。鉴定了 STEMI 和 NSTEMI 之间差异表达基因（DEGs），随后进行功能分析。进一步鉴定免疫相关 DEGs。选择一些免疫相关 DEGs 使用实时 PCR 进行表达验证。STEMI 和 NSTEMI 之间的免疫细胞存在显著差异，包括激活的树突状细胞、记忆 CD4 T 细胞、肥大细胞和 CD8 T 细胞。共鉴定出 229 个 DEGs，其功能与炎症调节和药物代谢有关。共鉴定出 21 个免疫相关 DEGs，它们可能在 STEMI 和 NSTEMI 中发挥重要作用。在 21 个免疫相关 DEGs 中，CCL18、NRP2、CXCR2、CXCL9、KIR2DL4、BPIFB1 和 IL33 等基因与免疫细胞显著相关，并且在 STEMI 和 NSTEMI 患者之间存在差异表达的趋势。我们的研究揭示了 STEMI 和 NSTEMI 之间免疫细胞亚群分布的差异，突出了关键的免疫相关基因及其与免疫细胞的关联，这可能为 AMI 的治疗提供新的思路。
