Remke Marianne, Groll Tanja, Metzler Thomas, Urbauer Elisabeth, Kövilein Janine, Schnalzger Theresa, Ruland Jürgen, Haller Dirk, Steiger Katja
Institute of Pathology, School of Medicine and Health, Technical University of Munich, Trogerstr. 18, 81675 Munich, Germany; Comparative Experimental Pathology, School of Medicine and Health, Technical University of Munich, Trogerstr. 18, 81675 Munich, Germany; Member of the German Cancer Consortium (DKTK), Partner Site Munich, Munich, Germany.
Institute of Pathology, School of Medicine and Health, Technical University of Munich, Trogerstr. 18, 81675 Munich, Germany; Comparative Experimental Pathology, School of Medicine and Health, Technical University of Munich, Trogerstr. 18, 81675 Munich, Germany.
Exp Mol Pathol. 2024 Dec;140:104938. doi: 10.1016/j.yexmp.2024.104938. Epub 2024 Oct 16.
Histomorphology is a powerful and cost-efficient tool for evaluating inflammatory and neoplastic conditions. Inflammatory bowel disease (IBD) is a widespread condition with globally rising incidences, and a lot of research is done to better understand the pathogenesis of IBD and to identify potential therapeutic approaches. However, standardized and reproducible scores for the histomorphological evaluation of murine IBD models are lacking. Therefore, we aimed to develop an easy-to-use and reproducible score for standardized assessment of colitis and associated cancer models.
In this study, samples from three different colitis models with and without associated cancer formation were analyzed to develop a universal, robust, and reproducible score for the grading of murine colitis models using the following three parameters: 1. Extent of leucocyte infiltration, 2. Tissue damage, 3. Architectural disruption of the mucosa.
A scoring system was established for different kinds of colitis models (genetically induced enterocolitis, genetically induced metabolic injury, and chemically induced colitis-associated cancer) and all stages of the disease, from mild inflammatory changes to severe inflammation with neoplastic changes as the extreme extent of IBD. The scoring scheme is easy to use, can easily be learned, and proves to have a high interrater reliability.
We propose a robust histological scoring system for the assessment of murine colitis and colitis-associated cancer models, giving more researchers access to conclusive and reliable histological assessment.
组织形态学是评估炎症和肿瘤性疾病的一种强大且经济高效的工具。炎症性肠病（IBD）是一种广泛存在且全球发病率不断上升的疾病，人们开展了大量研究以更好地理解IBD的发病机制并确定潜在的治疗方法。然而，目前缺乏用于小鼠IBD模型组织形态学评估的标准化且可重复的评分系统。因此，我们旨在开发一种易于使用且可重复的评分系统，用于对结肠炎及相关癌症模型进行标准化评估。
在本研究中，我们分析了来自三种不同的结肠炎模型（伴有或不伴有相关癌症形成）的样本，以使用以下三个参数开发一种通用、稳健且可重复的小鼠结肠炎模型分级评分系统：1. 白细胞浸润程度；2. 组织损伤；3. 黏膜结构破坏。
针对不同类型的结肠炎模型（基因诱导的小肠结肠炎、基因诱导的代谢损伤以及化学诱导的结肠炎相关癌症）以及疾病的所有阶段，从轻度炎症变化到伴有肿瘤变化的严重炎症（作为IBD的极端情况），建立了一种评分系统。该评分方案易于使用，容易掌握，并且具有较高的评分者间可靠性。
我们提出了一种用于评估小鼠结肠炎及结肠炎相关癌症模型的稳健的组织学评分系统，使更多研究人员能够进行结论性且可靠的组织学评估。
