Dai Yuhao, Li Jixiu, Wang Zhenzhen, Yang Shaoqi, Xiao Qingju, Gao Zipeng, Zhang Fengjing, Zhao Chenran, Yang Liang, Chen Shaopeng, Ding Wei
Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing, 400715, China.
China Tobacco Corporation Chongqing Tobacco Company, Chongqing, 400000, China.
AMB Express. 2024 Oct 17;14(1):116. doi: 10.1186/s13568-024-01760-x.
Tobacco bacterial wilt is a major limiting factor for tobacco production and development, and it is more likely to occur under perennial single cropping of tobacco. In recent years, the rotation of tobacco-radish has gradually become popular. Therefore, we studied the effects of years of tobacco-radish rotation on tobacco bacterial wilt occurrence and rhizosphere microorganisms. The results indicated that both SY and TY could significantly reduce the risk of tobacco bacterial wilt occurrence, and SY had the lowest disease index. The rotation of radish plants significantly increased the soil pH but decreased the contents of alkali-hydrolysed nitrogen and organic matter in the soil. Alkali-hydrolysed nitrogen and pH are the key factors affecting the composition of the bacterial community. Furthermore, radish rotation changed the composition of the soil microbial community, increased the diversity of the bacterial community, and significantly altered the bacterial community structure. At the genus level, the abundance of Sphingomonas species negatively correlated with Ralstonia increased significantly, while the relative abundance of Rhodanobacter species positively correlated with Ralstonia decreased significantly. Disease index, pH and available phosphorus were the main factors affecting the variation in different bacterial genera. The network analysis results showed that Ralstonia was less connected in the network than in the CK group, and the SY treatment group had a more complex bacterial network structure. Overall, 2 years of tobacco and radish rotation improved the bacterial community structure of the rhizosphere soil and alleviated the harm caused by tobacco bacterial wilt, which is highly important for the stability and health of the rhizosphere soil ecosystem.
烟草青枯病是烟草生产和发展的主要限制因素，在烟草连作多年的情况下更容易发生。近年来，烟草-萝卜轮作逐渐流行起来。因此，我们研究了多年烟草-萝卜轮作对烟草青枯病发生及根际微生物的影响。结果表明，SY和TY均能显著降低烟草青枯病发生风险，且SY的病情指数最低。萝卜种植轮作显著提高了土壤pH值，但降低了土壤中碱解氮和有机质含量。碱解氮和pH值是影响细菌群落组成的关键因素。此外，萝卜轮作改变了土壤微生物群落组成，增加了细菌群落多样性，并显著改变了细菌群落结构。在属水平上，与青枯菌呈负相关的鞘氨醇单胞菌属丰度显著增加，而与青枯菌呈正相关的红杆菌属相对丰度显著降低。病情指数、pH值和有效磷是影响不同细菌属变化的主要因素。网络分析结果表明，青枯菌在网络中的连接性比CK组低，SY处理组的细菌网络结构更复杂。总体而言，两年的烟草和萝卜轮作改善了根际土壤细菌群落结构，减轻了烟草青枯病造成的危害，这对根际土壤生态系统的稳定性和健康非常重要。
