• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Toll样受体4（TLR4）通过ERK-NF-κB通路促进坐骨神经损伤后背根神经节的再生与修复。

Toll-Like Receptor 4 (TLR4) Promotes DRG Regeneration and Repair after Sciatic Nerve Injury via the ERK-NF-kB Pathway.

作者信息

Xia Yiming, Yao Yi, Feng Yumei, Zhou Yiyue, Jiang Maorong, Ding Zihan, Qian Jiaxi, Bai Huiyuan, Cai Min, Yao Dengbing

机构信息

Medical School of Nantong University, No. 19 Qixiu Road, Nantong, Jiangsu, 226001, People's Republic of China.

School of Public Health, Nantong University, Nantong, Jiangsu, 226019, People's Republic of China.

出版信息

Mol Neurobiol. 2025 Apr;62(4):4172-4189. doi: 10.1007/s12035-024-04483-z. Epub 2024 Oct 17.

DOI:10.1007/s12035-024-04483-z
PMID:39420131
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11880167/
Abstract

Previously, we found that the expression of Toll-like receptor 4 (TLR4) is altered after sciatic nerve injury, and its differential expression plays a key role in recovery. However, the mechanisms by which TLR4 affects neuronal function in the dorsal root ganglion (DRG) have not been completely evaluated. The objective is to determine TLR4 expression in DRG tissues after sciatic neural injury and exploring the effects of TLR4 knockdown and overexpression in the DRG on neuronal function and nerve regeneration in rats in vivo and in vitro. We established a model of nerve injury and utilized molecular biology and cell biology experiments to explore the molecular mechanisms by which TLR4 in the DRG affects sciatic nerve restoration and regeneration after injury. Verified the localization of TLR4 in DRG neurons. Investigated pathways that related to apoptosis or nerve regeneration by which TLR4 regulates the function of DRG neurons. TLR4 expression was upregulated in the DRG tissues of rats after sciatic nerve injury. TLR4 overexpression promoted axon regeneration and inhibited apoptosis in DRG neurons. TLR4 promoted the regeneration of axons and the recovery of motor and sensory functions in the sciatic nerve after injury in vivo, and the data showed that TLR4 may regulate the function of DRG neurons and promote nerve repair and regeneration through the ERK and NF-κB signaling pathways in vivo and ex vivo. The study suggests that TLR4 may regulate the function of DRG neurons and promote nerve regeneration by affecting the ERK and NF-κB signaling pathways.

摘要

此前，我们发现坐骨神经损伤后Toll样受体4（TLR4）的表达发生改变，其差异表达在恢复过程中起关键作用。然而，TLR4影响背根神经节（DRG）神经元功能的机制尚未得到全面评估。目的是确定坐骨神经损伤后DRG组织中TLR4的表达，并探讨在体内和体外敲低和过表达DRG中的TLR4对大鼠神经元功能和神经再生的影响。我们建立了神经损伤模型，并利用分子生物学和细胞生物学实验来探索DRG中的TLR4影响损伤后坐骨神经修复和再生的分子机制。验证了TLR4在DRG神经元中的定位。研究了TLR4调节DRG神经元功能的与细胞凋亡或神经再生相关的途径。坐骨神经损伤后大鼠DRG组织中TLR4表达上调。TLR4过表达促进DRG神经元轴突再生并抑制细胞凋亡。在体内，TLR4促进损伤后坐骨神经轴突再生以及运动和感觉功能的恢复，数据表明TLR4可能在体内和体外通过ERK和NF-κB信号通路调节DRG神经元的功能并促进神经修复和再生。该研究表明，TLR4可能通过影响ERK和NF-κB信号通路来调节DRG神经元的功能并促进神经再生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/083819e9a013/12035_2024_4483_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/b10ae7b35f68/12035_2024_4483_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/8e544495f569/12035_2024_4483_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/10dd95e67b61/12035_2024_4483_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/1cf8a1d3a8e7/12035_2024_4483_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/de30751c4d36/12035_2024_4483_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/3fb9cf2dbdc9/12035_2024_4483_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/5a9b224bd4ee/12035_2024_4483_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/083819e9a013/12035_2024_4483_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/b10ae7b35f68/12035_2024_4483_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/8e544495f569/12035_2024_4483_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/10dd95e67b61/12035_2024_4483_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/1cf8a1d3a8e7/12035_2024_4483_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/de30751c4d36/12035_2024_4483_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/3fb9cf2dbdc9/12035_2024_4483_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/5a9b224bd4ee/12035_2024_4483_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8006/11880167/083819e9a013/12035_2024_4483_Fig8_HTML.jpg

相似文献

1
Toll-Like Receptor 4 (TLR4) Promotes DRG Regeneration and Repair after Sciatic Nerve Injury via the ERK-NF-kB Pathway.Toll样受体4（TLR4）通过ERK-NF-κB通路促进坐骨神经损伤后背根神经节的再生与修复。
Mol Neurobiol. 2025 Apr;62(4):4172-4189. doi: 10.1007/s12035-024-04483-z. Epub 2024 Oct 17.
2
The Exploratory Study of the PTEN-AKT/mTOR Signaling Pathway in the Corresponding Dorsal Root Ganglion during Compensatory Repair via Small Gap Amplification in Sciatic Nerve Injury.PTEN-AKT/mTOR 信号通路在坐骨神经损伤小间隙扩增后相应背根神经节代偿修复中的探索性研究。
J Integr Neurosci. 2024 Aug 21;23(8):157. doi: 10.31083/j.jin2308157.
3
Inhibitory Injury Signaling Represses Axon Regeneration After Dorsal Root Injury.背根损伤后，抑制性损伤信号会抑制轴突再生。
Mol Neurobiol. 2016 Sep;53(7):4596-605. doi: 10.1007/s12035-015-9397-6. Epub 2015 Aug 23.
4
Augmented Buyang Huanwu Decoction facilitates axonal regeneration after peripheral nerve transection through the regulation of inflammatory cytokine production.加味补阳还五汤通过调节炎症细胞因子的产生促进周围神经横断后的轴突再生。
J Ethnopharmacol. 2020 Oct 5;260:113063. doi: 10.1016/j.jep.2020.113063. Epub 2020 Jun 4.
5
Dynamic changes in Robo2 and Slit1 expression in adult rat dorsal root ganglion and sciatic nerve after peripheral and central axonal injury.成年大鼠外周和中枢轴突损伤后背根神经节和坐骨神经中Robo2和Slit1表达的动态变化
Neurosci Res. 2006 Nov;56(3):314-21. doi: 10.1016/j.neures.2006.07.014. Epub 2006 Sep 18.
6
The long noncoding RNA Arrl1 inhibits neurite outgrowth by functioning as a competing endogenous RNA during neuronal regeneration in rats.长链非编码 RNA Arrl1 通过在大鼠神经元再生过程中作为竞争性内源性 RNA 发挥作用，抑制轴突生长。
J Biol Chem. 2020 Jun 19;295(25):8374-8386. doi: 10.1074/jbc.RA119.011917. Epub 2020 Apr 26.
7
The Intrinsic Neuronal Activation of the CXCR4 Signaling Axis Is Associated with a Pro-Regenerative State in Cervical Primary Sensory Neurons Conditioned by a Sciatic Nerve Lesion.CXCR4信号轴的内在神经元激活与坐骨神经损伤所调节的颈初级感觉神经元中的促再生状态相关。
Int J Mol Sci. 2024 Dec 29;26(1):193. doi: 10.3390/ijms26010193.
8
Electroacupuncture inhibits TLR4/NF-κB signaling in the dorsal root ganglion of rats with spared nerve injury.电针对 spared nerve injury 大鼠背根神经节 TLR4/NF-κB 信号通路的抑制作用。
Acupunct Med. 2024 Oct;42(5):275-284. doi: 10.1177/09645284241279874. Epub 2024 Sep 27.
9
Comparison of changes in beta-tubulin and NF gene expression in rat DRG neurons under regeneration-permissive and regeneration-prohibitive conditions.再生许可和再生抑制条件下大鼠背根神经节神经元中β-微管蛋白和NF基因表达变化的比较。
Brain Res. 1994 Feb 21;637(1-2):233-41. doi: 10.1016/0006-8993(94)91238-6.
10
Most dorsal root ganglion neurons of the adult rat survive nerve crush injury.成年大鼠的大多数背根神经节神经元在神经挤压伤后存活下来。
Somatosens Mot Res. 1995;12(3-4):177-89. doi: 10.3109/08990229509093656.

引用本文的文献

1
From bench to bedside: nanomedicine development for intracerebral hemorrhage - exploring microenvironment, innovation, and translation.从实验室到临床：脑出血的纳米医学发展——探索微环境、创新与转化
J Nanobiotechnology. 2025 Aug 14;23(1):567. doi: 10.1186/s12951-025-03661-y.
2
Gut microbiota-mediated pain sensitization: mechanisms and therapeutic implications.肠道微生物群介导的疼痛敏化：机制与治疗意义。
Front Pain Res (Lausanne). 2025 Jul 3;6:1626515. doi: 10.3389/fpain.2025.1626515. eCollection 2025.
3
Identification of pivotal genes and regulatory networks associated with SAH based on multi-omics analysis and machine learning.

本文引用的文献

1
Comparative Proteomics Analysis of Growth-Primed Adult Dorsal Root Ganglia Reveals Key Molecular Mediators for Peripheral Nerve Regeneration.生长启动的成年背根神经节的比较蛋白质组学分析揭示了外周神经再生的关键分子介质。
eNeuro. 2023 Jan 5;10(1). doi: 10.1523/ENEURO.0168-22.2022. Print 2023 Jan.
2
Effects of regenerative peripheral nerve interface on dorsal root ganglia neurons following peripheral axotomy.周围神经再生接口对周围神经切断术后背根神经节神经元的影响。
Front Neurosci. 2022 Sep 7;16:914344. doi: 10.3389/fnins.2022.914344. eCollection 2022.
3
GAP-43 and BASP1 in Axon Regeneration: Implications for the Treatment of Neurodegenerative Diseases.
基于多组学分析和机器学习识别与蛛网膜下腔出血相关的关键基因和调控网络。
Sci Rep. 2025 Apr 24;15(1):14401. doi: 10.1038/s41598-025-98754-x.
生长相关蛋白43和脑酸性可溶性蛋白1在轴突再生中的作用：对神经退行性疾病治疗的启示
Front Cell Dev Biol. 2020 Sep 3;8:567537. doi: 10.3389/fcell.2020.567537. eCollection 2020.
4
Expression of Toll-like receptors 4 and 7 in murine peripheral nervous system development.Toll 样受体 4 和 7 在鼠外周神经系统发育中的表达。
Ann Anat. 2020 Sep;231:151526. doi: 10.1016/j.aanat.2020.151526. Epub 2020 May 4.
5
Unique Characteristics of the Dorsal Root Ganglion as a Target for Neuromodulation.背根神经节作为神经调节靶点的独特特征。
Pain Med. 2019 Jun 1;20(Suppl 1):S23-S30. doi: 10.1093/pm/pnz012.
6
Promotion of Peripheral Nerve Regeneration by Stimulation of the Extracellular Signal-Regulated Kinase (ERK) Pathway.刺激细胞外信号调节激酶（ERK）通路促进周围神经再生。
Anat Rec (Hoboken). 2019 Aug;302(8):1261-1267. doi: 10.1002/ar.24126. Epub 2019 Apr 22.
7
Interleukin-6 contributes to initiation of neuronal regeneration program in the remote dorsal root ganglia neurons after sciatic nerve injury.白细胞介素-6有助于坐骨神经损伤后远隔背根神经节神经元启动神经元再生程序。
Histochem Cell Biol. 2019 Aug;152(2):109-117. doi: 10.1007/s00418-019-01779-3. Epub 2019 Mar 29.
8
Toll-Like Receptor 4 (TLR4) Expression Affects Schwann Cell Behavior in vitro.Toll 样受体 4（TLR4）表达影响雪旺细胞的体外行为。
Sci Rep. 2018 Jul 25;8(1):11179. doi: 10.1038/s41598-018-28516-5.
9
The intriguing nature of dorsal root ganglion neurons: Linking structure with polarity and function.背根神经节神经元的奇妙特性：结构与极性和功能的联系。
Prog Neurobiol. 2018 Sep;168:86-103. doi: 10.1016/j.pneurobio.2018.05.002. Epub 2018 May 2.
10
Roles of toll-like receptors: From inflammation to lung cancer progression.Toll样受体的作用：从炎症到肺癌进展。
Biomed Rep. 2018 Feb;8(2):126-132. doi: 10.3892/br.2017.1034. Epub 2017 Dec 28.