Sheng Xin-Yuan, Zhang Hai-Jiang, Chen Xin-Juan, Wang Kui-Wu
School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, P. R. China.
Jiangsu Key Laboratory of Regional Specific Resource Pharmaceutical Transformation, Huaiyin Institute of Technology, Huai'an, 223003, P. R. China.
Chem Biodivers. 2025 Mar;22(3):e202402204. doi: 10.1002/cbdv.202402204. Epub 2024 Nov 13.
This study reported an effective method for the degradation of Chieh-qua (Benincasa hispida var. Chieh-qua How) polysaccharides (BHCP) by a hydrogen peroxide-ascorbic acid oxidation (HO-V) system. The degradation conditions were optimized using a Box-Behnken response surface design as concentration of HO-V 19.5 mM, degradation temperature 46.4 °C and degradation time 1.0 h. The average molecular weight was decreased and total sugar content was raised of the degraded polysaccharide (DBHCP). Two refined degraded polysaccharides (DBHCP-1, DBHCP-2) were purified and prepared, and their structures were analyzed by chemical and spectral analysis. The in vitro experiments showed that degraded polysaccharides (DBHCP and DBHCP-1) have better antioxidant and anti-tyrosinase activity than natural polysaccharide BHCP. These findings support the potential application of Chieh-qua polysaccharides in the food and medical industries.
本研究报道了一种用过氧化氢-抗坏血酸氧化（HO-V）体系降解节瓜（Benincasa hispida var. Chieh-qua How）多糖（BHCP）的有效方法。采用Box-Behnken响应面设计对降解条件进行优化，得到HO-V浓度为19.5 mM、降解温度为46.4 °C、降解时间为1.0 h。降解后的多糖（DBHCP）平均分子量降低，总糖含量升高。纯化制备了两种精制降解多糖（DBHCP-1、DBHCP-2），并通过化学和光谱分析对其结构进行了分析。体外实验表明，降解多糖（DBHCP和DBHCP-1）比天然多糖BHCP具有更好的抗氧化和抗酪氨酸酶活性。这些发现支持了节瓜多糖在食品和医药行业的潜在应用。
