Wang Min, Yang Songguang, Liu Wei, Cao Zhenqiang, Chen Lin, Liu Wenrui, Xie Dasen, Yan Jinqiang, Jiang Biao, Peng Qingwu
Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Guangdong Key Laboratory for New Technology Research of Vegetables, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Front Plant Sci. 2023 Apr 20;14:1158735. doi: 10.3389/fpls.2023.1158735. eCollection 2023.
Gynoecy demonstrates an earlier production of hybrids and a higher yield and improves the efficiency of hybrid seed production. Therefore, the utilization of gynoecy is beneficial for the genetic breeding of chieh-qua. However, little knowledge of gynoecious-related genes in chieh-qua has been reported until now. Here, we used an F population from the cross between the gynoecious line 'A36' and the monoecious line 'SX' for genetic mapping and revealed that chieh-qua gynoecy was regulated by a single recessive gene. We fine-mapped it into a 530-kb region flanked by the markers Indel-3 and KASP145 on Chr.8, which harbors eight candidate genes. One of the candidate genes, , encoding networked protein 4 (), contained a non-synonymous SNP resulting in the amino acid substitution of isoleucine (ATA; I) to methionine (ATG; M). was prominently expressed in the female flower, and only three genes related to ethylene synthesis were significantly expressed between 'A36' and 'SX.' The results presented here provide support for the as the most likely candidate gene for chieh-qua gynoecy, which differed from the reported gynoecious genes.
雌性系表现出较早产生杂种且产量较高，提高了杂交种子生产效率。因此，利用雌性系有利于节瓜的遗传育种。然而，迄今为止，关于节瓜中与雌性相关基因的了解甚少。在此，我们利用雌性系‘A36’与雌雄同株系‘SX’杂交产生的F群体进行基因定位，发现节瓜的雌性受一个单隐性基因调控。我们将其精细定位到8号染色体上标记Indel-3和KASP145侧翼的一个530 kb区域，该区域包含8个候选基因。其中一个候选基因，编码网络蛋白4（），包含一个非同义单核苷酸多态性，导致异亮氨酸（ATA；I）到蛋氨酸（ATG；M）的氨基酸替换。在雌花中显著表达，并且在‘A36’和‘SX’之间只有三个与乙烯合成相关的基因显著表达。此处呈现的结果支持作为节瓜雌性最可能的候选基因，这与已报道的雌性基因不同。
