Peng Jiazhu, Gao Yin, Qiao Yanchun, Wang Guoping
Vegetable Research Science Department, Guangzhou Academy of Agricultural and Rural Sciences, Guangzhou, China.
Vegetable Research Institute, Guangzhou Academy of Agricultural Sciences, Guangzhou, China.
Front Plant Sci. 2024 Oct 21;15:1388115. doi: 10.3389/fpls.2024.1388115. eCollection 2024.
Flesh color is an essential trait in chieh-qua ( Cogn. var. How); however, the inheritance and molecular basis of green flesh trait remain unclear.
In the present study, two F populations, derived from 1742 (white flesh) × FJ3211 (green flesh) and J16 (white flesh) × FJ5 (green flesh), were used to identify the green flesh () locus.
Genetic analysis revealed that the presence of green flesh was a quantitative trait that closely followed a normal distribution. Combining the results from QTL mapping and BSA-seq analysis, the locus was preliminarily determined to be located on chromosome 05 and was narrowed down to a 2.55-Mb interval by linkage analysis. A large J16 × FJ5 F population comprising 3,180 individuals was subsequently used to screen the recombinants, and the locus was fine-mapped to a region of 329.70 kb that harbors six genes. One of the candidate genes, , the zinc-finger transcription factor LOL1 (lsd one like 1 protein; ), was the strongest candidate gene for the locus according to sequence variation and expression analysis. Additionally, a point mutation (A > C) in resulted in the substitution of threonine (T) with proline (P) in the amino acid sequence, showing a complete relationship linked with flesh color in a panel of 45 germplasms.
The study suggests that promotes the accumulation of chlorophyll content in chieh-qua and lead to green flesh. Our findings establish a theoretical and technical foundation for breeding different flesh color lines and elucidating the underlying mechanisms of flesh color in chieh-qua.
瓜肉颜色是节瓜（Cogn. var. How）的一个重要性状；然而，绿肉性状的遗传和分子基础仍不清楚。
在本研究中，使用了两个F群体，分别来自1742（白肉）×FJ3211（绿肉）和J16（白肉）×FJ5（绿肉），来鉴定绿肉（）位点。
遗传分析表明，绿肉的存在是一个遵循正态分布的数量性状。结合QTL定位和BSA-seq分析结果，初步确定该位点位于5号染色体上，并通过连锁分析将其缩小到一个2.55 Mb的区间。随后，使用一个由3180个个体组成的大的J16×FJ5 F群体来筛选重组体，该位点被精细定位到一个329.70 kb的区域，该区域包含6个基因。候选基因之一，锌指转录因子LOL1（类lsd1蛋白；），根据序列变异和表达分析，是该位点最有可能的候选基因。此外，中的一个点突变（A>C）导致氨基酸序列中的苏氨酸（T）被脯氨酸（P）取代，在一组45份种质资源中显示出与瓜肉颜色完全相关。
该研究表明，促进节瓜中叶绿素含量的积累并导致绿肉。我们的研究结果为培育不同瓜肉颜色的品系和阐明节瓜瓜肉颜色的潜在机制奠定了理论和技术基础。
