Departamento de Saúde Universidade Estadual de Santa Cruz IlhéusBA Brasil Departamento de Saúde, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
Programa Saúde da Família SalvadorBA Brasil Programa Saúde da Família, Salvador, BA, Brasil.
Arch Endocrinol Metab. 2024 Aug 13;68:e230248. doi: 10.20945/2359-4292-2023-0248. eCollection 2024.
To analyze in silico the evidence of molecular mimicry between human beta-cell autoantigens and cow's milk proteins as a potential type 1 diabetes mellitus (T1DM) trigger.
The in silico analysis was performed using bioinformatics tools to compare the amino acid sequences of cow's milk proteins (bovine serum albumin [BSA] and beta-lactoglobulin [BLG]) and human beta-cell autoantigens (glutamic acid decarboxylase-65 [GAD-65], insulin, and zinc transporter 8 [ZnT8]). The structural and functional characteristics of the proteins were analyzed to identify potential molecular mimicry mechanisms.
The results of the in silico analysis showed significant sequence similarity between BSA/BLG and GAD-65/human insulin/ZnT8, ranging from 19.64% to 27.27%. The cow's milk proteins evaluated shared structural features with the beta-cell antigens selected for comparison, indicating a potential for molecular mimicry between these proteins.
The findings of this study provide further evidence for a potential role of cow's milk proteins in triggering T1DM. The in silico analysis suggests that molecular mimicry mechanisms between cow's milk proteins and human beta-cell antigens may contribute to the autoimmune response leading to T1DM.
通过计算机模拟分析人类胰岛自身抗原与牛奶蛋白之间的分子模拟现象，探讨其作为 1 型糖尿病（T1DM）发病诱因的可能性。
采用生物信息学工具进行计算机模拟分析，比较牛奶蛋白（牛血清白蛋白[BSA]和β-乳球蛋白[BLG]）和人类胰岛自身抗原（谷氨酸脱羧酶-65[GAD-65]、胰岛素和锌转运蛋白 8[ZnT8]）的氨基酸序列。分析蛋白质的结构和功能特征，以确定潜在的分子模拟机制。
计算机模拟分析结果显示，BSA/BLG 与 GAD-65/人胰岛素/ZnT8 之间的序列相似度显著，范围为 19.64%至 27.27%。评估的牛奶蛋白与所选比较的β细胞抗原具有相似的结构特征，表明这些蛋白之间可能存在分子模拟现象。
本研究的发现进一步支持了牛奶蛋白在触发 T1DM 中的潜在作用。计算机模拟分析提示，牛奶蛋白与人类胰岛自身抗原之间的分子模拟机制可能参与了导致 T1DM 的自身免疫反应。
