Otaru Nize, Pugin Benoît, Plüss Serafina, Hojsak Iva, Braegger Christian, Lacroix Christophe
Nutrition Research Unit, University Children's Hospital Zürich, Zürich 8032, Switzerland.
Laboratory of Food Biotechnology, Department of Health Sciences and Technology (HEST), ETH Zürich, Zürich 8092, Switzerland.
Microbiome Res Rep. 2024 Jun 4;3(3):32. doi: 10.20517/mrr.2023.75. eCollection 2024.
Gut microbial features and the role of early life stress in pediatric functional abdominal pain-not otherwise specified (FAP-NOS) have never been investigated before. Here, we hypothesize that early life stress is more prevalent in FAP-NOS compared to healthy controls and that fecal microbial profiles and related metabolites differ between groups. In an international multicenter case-control study, FAP-NOS patients ( = 40) were compared to healthy controls ( = 55). Stool samples and demographic and clinical data including early life traumatic events and antibiotics treatments were collected from children aged four to twelve years. Fecal microbial profiles were assessed with 16S rRNA gene amplicon sequencing. Microbial metabolite concentrations in fecal supernatant, including short-chain fatty acids and amino acids, were detected liquid chromatography. Microbial richness was increased in FAP-NOS compared to healthy controls and microbial composition (unweighted UniFrac) differed between groups. Three distinct amplicon sequencing variants and two distinct species were enriched in FAP-NOS compared to controls, with no observed changes at higher taxonomic levels. No differences in microbial metabolites and early life stress were observed between groups. The presented hypothesis could not be proven, with no observed differences in occurrence of early life stress, and fecal microbial metabolic profiles between pediatric FAP-NOS and healthy controls. Pediatric FAP-NOS patients exhibited mild differences in the fecal microbial community compared with controls. Further large-scale studies with high-resolution techniques are warranted to address the biological relevance of present observations.
肠道微生物特征以及早期生活应激在未另作说明的小儿功能性腹痛（FAP-NOS）中的作用此前从未被研究过。在此，我们假设与健康对照相比，早期生活应激在FAP-NOS中更为普遍，并且两组之间的粪便微生物谱及相关代谢物存在差异。在一项国际多中心病例对照研究中，将40例FAP-NOS患者与55例健康对照进行比较。收集了4至12岁儿童的粪便样本以及人口统计学和临床数据，包括早期生活创伤事件和抗生素治疗情况。通过16S rRNA基因扩增子测序评估粪便微生物谱。采用液相色谱法检测粪便上清液中的微生物代谢物浓度，包括短链脂肪酸和氨基酸。与健康对照相比，FAP-NOS患者的微生物丰富度增加，且两组之间的微生物组成（非加权UniFrac）存在差异。与对照组相比，FAP-NOS中三种不同的扩增子测序变体和两种不同的物种富集，在更高分类水平上未观察到变化。两组之间在微生物代谢物和早期生活应激方面未观察到差异。所提出的假设未得到证实，小儿FAP-NOS与健康对照在早期生活应激的发生率以及粪便微生物代谢谱方面未观察到差异。与对照组相比，小儿FAP-NOS患者的粪便微生物群落存在轻微差异。有必要开展进一步的大规模高分辨率技术研究，以探讨当前观察结果的生物学相关性。
