Cui Mengni, Trimigno Alessia, Castro-Mejía Josue L, Reitelseder Søren, Bülow Jacob, Bechshøft Rasmus Leidesdorff, Nielsen Dennis Sandris, Holm Lars, Engelsen Søren Balling, Khakimov Bekzod
Chemometrics and Analytical Technology Section, Department of Food Science University of Copenhagen Rolighedsvej 26, 1958 Frederiksberg C, Denmark.
Food Microbiology & Fermentation Section, Department of Food Science University of Copenhagen Rolighedsvej 26, 1958 Frederiksberg C, Denmark.
Metabolites. 2021 Oct 21;11(11):717. doi: 10.3390/metabo11110717.
This study investigated how body mass index (BMI), physical fitness, and blood plasma lipoprotein levels are related to the fecal metabolome in older adults. The fecal metabolome data were acquired using proton nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry on 163 healthy older adults (65-80 years old, 80 females and 83 males). Overweight and obese subjects (BMI ≥ 27) showed higher levels of fecal amino acids (AAs) (valine, alanine, and phenylalanine) compared to normal-weight subjects (BMI ≤ 23.5). Adults classified in the high-fitness group displayed slightly lower concentrations of fecal short-chain fatty acids, propionic acid, and AAs (methionine, leucine, glutamic acid, and threonine) compared to the low-fitness group. Subjects with lower levels of cholesterol in low-density lipoprotein particles (LDL ≤2.6 mmol/L) displayed higher fecal levels of valine, glutamic acid, phenylalanine, and lactic acid, while subjects with a higher level of cholesterol in high-density lipoprotein particles (HDL ≥2.1 mmol/L) showed lower fecal concentration of isovaleric acid. The results from this study suggest that the human fecal metabolome, which primarily represents undigested food waste and metabolites produced by the gut microbiome, carries important information about human health and should be closely integrated to other data for a better understanding of the role of the gut microbiome and diet on human health and metabolism.
本研究调查了体重指数（BMI）、身体素质和血浆脂蛋白水平与老年人粪便代谢组之间的关系。对163名健康老年人（65 - 80岁，80名女性和83名男性）使用质子核磁共振波谱和气相色谱 - 质谱联用技术获取粪便代谢组数据。超重和肥胖受试者（BMI≥27）与正常体重受试者（BMI≤23.5）相比，粪便氨基酸（AAs）（缬氨酸、丙氨酸和苯丙氨酸）水平更高。与低身体素质组相比，高身体素质组成年人的粪便短链脂肪酸、丙酸和AAs（蛋氨酸、亮氨酸、谷氨酸和苏氨酸）浓度略低。低密度脂蛋白颗粒中胆固醇水平较低的受试者（LDL≤2.6 mmol/L）粪便中缬氨酸、谷氨酸、苯丙氨酸和乳酸水平较高，而高密度脂蛋白颗粒中胆固醇水平较高的受试者（HDL≥2.1 mmol/L）粪便中异戊酸浓度较低。本研究结果表明，人类粪便代谢组主要代表未消化的食物残渣和肠道微生物群产生的代谢产物，携带着有关人类健康的重要信息，应与其他数据紧密整合，以更好地理解肠道微生物群和饮食对人类健康与代谢的作用。
