A2-165 metabolizes host- and media-derived chemicals and induces transcriptional changes in colonic epithelium in GuMI human gut microphysiological system.

Recently, a GuMI gut microphysiological system has been established to coculture oxygen-intolerant () A2-165 with organoids-derived primary human colonic epithelium. This study aims to test if this GuMI system applies to different donors with different healthy states and uses metabolomics to reveal the role of gut microbes in modulating host- and diet-derived molecules in the gut lumen. Organoids-derived colonic monolayers were generated from an uninflamed region of diverticulitis, ulcerative colitis, and Crohn's disease patients and then integrated into the GuMI system to coculture with A2-165 for 2 to 4 days. Apical media was collected for metabolomic analysis. Targeted metabolomics was performed to profile 169 polar chemicals under three conditions: conventional static culture without bacteria, GuMI without bacteria, and GuMI with . The barrier function of monolayers was measured using transepithelial resistance. GuMI successfully cocultured patient-derived monolayers and for up to 4 days, with active bacterial growth. Introducing flow and oxygen gradient significantly increases the barrier function, while exposure to slightly increases the barrier function. Targeted metabolomics screened 169 compounds and detected 76 metabolites, of which 70 significantly differed between at least two conditions. significantly modulates the levels of nucleosides, nucleobases, and amino acids on the apical side. Further analysis suggests that changes the mRNA level of 260 transcription factor genes in colonic epithelial cells. The GuMI physiomimetic system can maintain the coculture of and colonic epithelium from different donors. Together with metabolomics, we identified the modulation of in extracellular chemicals and colonic epithelial cell transcription in coculture with human colonic epithelium, which may reflect its function in gut lumen .